Parallel profiling of cancer cells and proteins using a graphene oxide functionalized ac-EHD SERS immunoassay

Nanoscale
K Kamil RezaMatt Trau

Abstract

Circulating biomarkers have emerged as promising non-invasive, real-time surrogates for cancer diagnosis, prognosis and monitoring of the therapeutic response. Current bio-sensing techniques mostly involve detection of either circulating cells or proteins which are inadequate in unfolding complex pathologic transformations. Herein, we report parallel detection of cellular and molecular markers (protein) for cancer using a multiplex platform featuring (i) graphene oxide (GO) functionalization that increases the active surface area and more importantly reduces the functionalization steps for rapid detection, (ii) alternating-current electrohydrodynamic (ac-EHD) fluid flow that provides delicate micro-mixing to enhance target-sensor interactions thereby minimizing non-specific binding and (iii) surface enhanced Raman scattering (SERS) for multiplex detection. We find that our platform possesses high sensitivity for detecting both proteins and cells. More importantly, this platform not only detects the cancer cells but also can simultaneously monitor the heterogeneous expression of cell surface proteins which could be clinically useful to determine effective patient therapy. We demonstrate the specific and sensitive detection of br...Continue Reading

References

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Jul 29, 2016·Scientific Reports·Abu Ali Ibn SinaMatt Trau
Mar 9, 2017·Chemical Reviews·Jian-Feng LiZhong-Qun Tian

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Citations

Mar 28, 2020·International Journal of Molecular Sciences·Maria Blanco-Formoso, Ramon A Alvarez-Puebla
Jan 19, 2021·ACS Biomaterials Science & Engineering·Zahra Mohammadpour, Keivan Majidzadeh-A

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Methods Mentioned

BETA
biopsy
flow cytometry
biosensing
biosensors
chip
Raman spectroscopy
scanning electron microscopy
MDA
fluorescence imaging
ELISA

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