Paramagnetic nanoparticles as a platform for FRET-based sarcosine picomolar detection

Scientific Reports
Zbynek HegerRene Kizek

Abstract

Herein, we describe an ultrasensitive specific biosensing system for detection of sarcosine as a potential biomarker of prostate carcinoma based on Förster resonance energy transfer (FRET). The FRET biosensor employs anti-sarcosine antibodies immobilized on paramagnetic nanoparticles surface for specific antigen binding. Successful binding of sarcosine leads to assembly of a sandwich construct composed of anti-sarcosine antibodies keeping the Förster distance (Ro) of FRET pair in required proximity. The detection is based on spectral overlap between gold-functionalized green fluorescent protein and antibodies@quantum dots bioconjugate (λex 400 nm). The saturation curve of sarcosine based on FRET efficiency (F₆₀₄/F₅₁₀ ratio) was tested within linear dynamic range from 5 to 50 nM with detection limit down to 50 pM. Assembled biosensor was then successfully employed for sarcosine quantification in prostatic cell lines (PC3, 22Rv1, PNT1A), and urinary samples of prostate adenocarcinoma patients.

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Citations

Jul 21, 2015·Biosensors & Bioelectronics·Maja StanisavljevicVojtech Adam
Feb 19, 2017·Biosensors & Bioelectronics·Jiri KudrVojtech Adam
May 6, 2019·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·C S PundirParveen Kumar
Apr 21, 2018·Journal of Nanobiotechnology·Hana BuchtelovaZbynek Heger
Aug 3, 2021·Analytical Biochemistry·Mahsa ImaniNosratollah Zarghami
Sep 29, 2018·ACS Applied Materials & Interfaces·Amitava MoulickVojtech Adam

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Methods Mentioned

BETA
biosensors
biosensing
scanning electron microscopy
biosensor
ELISA
FRET
xenograft
IEC
X-ray
electrochemical

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