Parameterizing the Transport Pathways for Cell Invasion in Complex Scaffold Architectures

Tissue Engineering. Part C, Methods
Jennifer Claire AshworthRuth Elizabeth Cameron

Abstract

Interconnecting pathways through porous tissue engineering scaffolds play a vital role in determining nutrient supply, cell invasion, and tissue ingrowth. However, the global use of the term "interconnectivity" often fails to describe the transport characteristics of these pathways, giving no clear indication of their potential to support tissue synthesis. This article uses new experimental data to provide a critical analysis of reported methods for the description of scaffold transport pathways, ranging from qualitative image analysis to thorough structural parameterization using X-ray Micro-Computed Tomography. In the collagen scaffolds tested in this study, it was found that the proportion of pore space perceived to be accessible dramatically changed depending on the chosen method of analysis. Measurements of % interconnectivity as defined in this manner varied as a function of direction and connection size, and also showed a dependence on measurement length scale. As an alternative, a method for transport pathway parameterization was investigated, using percolation theory to calculate the diameter of the largest sphere that can travel to infinite distance through a scaffold in a specified direction. As proof of principle, t...Continue Reading

References

Nov 9, 2002·Biomaterials·Angela S P LinRobert E Guldberg
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Mar 3, 2009·Biomaterials·Biman B Mandal, Subhas C Kundu
Mar 17, 2011·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Jie Liu, Klaus Regenauer-Lieb
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Jun 3, 2011·Tissue Engineering. Part C, Methods·Richard J MillsJustin J Cooper-White
Oct 19, 2011·Acta Biomaterialia·N DavidenkoR E Cameron
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Mar 4, 2014·Materials Science & Engineering. C, Materials for Biological Applications·K M PawelecR E Cameron
Apr 17, 2015·Advanced Healthcare Materials·Jennifer C AshworthRuth E Cameron

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Citations

Nov 6, 2018·Journal of Materials Science. Materials in Medicine·Jennifer C AshworthRuth E Cameron
May 6, 2020·Journal of Materials Science. Materials in Medicine·Giovanni S OffedduRuth E Cameron
Dec 9, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·K M PawelecR E Cameron
Nov 14, 2019·Materials·Jordi Caballé-SerranoDieter D Bosshardt

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