Parameters of human hepatitis delta virus genome replication: the quantity, quality, and intracellular distribution of viral proteins and RNA

Journal of Virology
S GudimaJohn M Taylor

Abstract

Assembly of hepatitis delta virus (HDV) in infected human hepatocytes involves association of the 1,679- nucleotide single-stranded genomic RNA (deltaRNA) with multiple copies of both small and large forms of the delta protein (deltaAg) to form a ribonucleoprotein particle which in turn interacts with envelope proteins of the natural helper virus, hepatitis B virus. Subsequently, for initiation of a new round of replication, the amount of small deltaAg within the assembled HDV particle is both necessary and sufficient. Quantitative assays were used in order to better understand just how much deltaAg is needed. The molar ratio of deltaAg species to genomic deltaRNA in assembled HDV particles was approximately 200. Next, this ratio was determined for cells under several different experimental situations in which HDV genome replication was occurring. These included replication in woodchuck liver and also in mouse liver and skeletal muscle, as well as replication in stably and transiently transfected cultured human hepatoblastoma cells. Surprisingly, in almost all these situations the molar ratios were comparable to that observed for HDV particles. This was true for different times after the initiation of replication and was indepe...Continue Reading

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Citations

Apr 16, 2013·Journal of Inorganic Biochemistry·Kamila Stokowa-SołtysMałgorzata Jeżowska-Bojczuk
Apr 23, 2003·Trends in Microbiology·John M Taylor
Nov 23, 2007·Journal of Virology·Jinhong ChangJohn Taylor
Aug 14, 2003·Journal of Virology·Jinhong Chang, John M Taylor
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Oct 22, 2018·Gastroenterology·Christopher KohJeffrey S Glenn

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