Paramyxovirus Sendai virus-like particle formation by expression of multiple viral proteins and acceleration of its release by C protein

Virology
Fumihiro SugaharaTakemasa Sakaguchi

Abstract

Envelope viruses maturate by macromolecule assembly and budding. To investigate these steps, we generated virus-like particles (VLPs) by co-expression of structural proteins of Sendai virus (SeV), a prototype of the family Paramyxoviridae. Simultaneous expression of matrix (M), nucleo- (N), fusion (F), and hemagglutinin-neuraminidase (HN) proteins resulted in the generation of VLPs that had morphology and density similar to those of authentic virus particles, although the efficiency of release from cells was significantly lower than that of the virus. By using this VLP formation as a model of virus budding, roles of individual proteins in budding were investigated. The M protein was a driving force of budding, and the F protein facilitated and the HN protein suppressed VLP release. Either of the glycoproteins, F or HN, as well as the N protein, significantly shifted density of VLPs to that of virus particles, suggesting that viral proteins bring about integrity of VLPs by protein-protein interactions. We further found that co-expression of a nonstructural protein, C, but not V, enhanced VLP release to a level comparable to that of virus particles, demonstrating that the C protein plays a role in virus budding.

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Citations

Jul 5, 2005·Journal of Virology·Takemasa SakaguchiTetsuya Yoshida
Sep 15, 2006·Journal of Virology·Homer D PantuaTrudy G Morrison
Nov 13, 2008·Virology Journal·Jared R PatchChristopher C Broder
Mar 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Victoria A MoneyRobert P Yeo
Feb 26, 2010·Journal of Virology·Lori W McGinnesTrudy G Morrison
Feb 25, 2011·Expert Reviews in Molecular Medicine·Hector C Aguilar, Benhur Lee
Mar 30, 2011·PloS One·Mary E PiperSonja R Gerrard
Apr 16, 2011·PloS One·Pramila WalpitaLinfa Wang
Nov 28, 2007·Uirusu·Takashi Irie, Takemasa Sakaguchi
Sep 21, 2010·Uirusu·Takashi Irie
Feb 12, 2011·Archives of Virology·Hiroshi FujitaYasufumi Kaneda
Apr 8, 2009·Expert Review of Vaccines·Joel R Haynes
Sep 21, 2005·Microbiology and Immunology·Hitoshi WatanabeTakemasa Sakaguchi
Oct 8, 2008·Immunological Reviews·Judith M FontanaPaul A Rota
Aug 20, 2011·Microbiology and Immunology·Takemasa SakaguchiRyoko Kawabata
Nov 26, 2009·Virology·Zifei PeiAnthony P Schmitt
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Mar 10, 2016·The Journal of General Virology·Megan K BrackenMichael A Hoffman
Sep 4, 2018·Expert Review of Vaccines·Braeden DonaldsonVernon K Ward
Sep 5, 2015·Journal of Virology·Kosuke OdaTakemasa Sakaguchi

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