Paraoxonase 2 is down-regulated by the Pseudomonas aeruginosa quorumsensing signal N-(3-oxododecanoyl)-L-homoserine lactone and attenuates oxidative stress induced by pyocyanin

The Biochemical Journal
S HorkeJohn F Teiber

Abstract

Two virulence factors produced by Pseudomonas aeruginosa are pyocyanin and N-(3-oxododecanoyl)-L-homoserine lactone (3OC12). Pyocyanin damages host cells by generating ROS (reactive oxygen species). 3OC12 is a quorum-sensing signalling molecule which regulates bacterial gene expression and modulates host immune responses. PON2 (paraoxonase-2) is an esterase that inactivates 3OC12 and potentially attenuates Ps. aeruginosa virulence. Because increased intracellular Ca2+ initiates the degradation of PON2 mRNA and protein and 3OC12 causes increases in cytosolic Ca2+, we hypothesized that 3OC12 would also down-regulate PON2. 3OC12 and the Ca2+ ionophore A23187 caused a rapid cytosolic Ca2+ influx and down-regulated PON2 mRNA, protein and hydrolytic activity in A549 and EA.hy 926 cells. The decrease in PON2 hydrolytic activity was much more extensive and rapid than decreases in protein, suggesting a rapid post-translational mechanism which blocks PON2's hydrolytic activity. The Ca2+ chelator BAPTA/AM [1,2-bis-(o-aminophenoxy)ethane-N,N,N',N'-tetra-acetic acid tetrakis(acetoxymethyl ester)] diminished the ability of 3OC12 to decrease PON2, demonstrating that the effects are mediated by Ca2+. PON2 also has antioxidative properties and ...Continue Reading

References

Jan 1, 1980·Journal of Bacteriology·H M Hassan, I Fridovich
Nov 24, 1998·Infection and Immunity·G M DenningB E Britigan
Feb 6, 2003·American Journal of Respiratory and Critical Care Medicine·Philippe LespritClaude Carbon
Nov 11, 2003·American Journal of Respiratory Cell and Molecular Biology·Robert AdamoAlice Prince
Jun 24, 2004·Infection and Immunity·Gee W LauDimitri Mavrodi
Oct 27, 2004·Infection and Immunity·Doreen S W HooiDavid I Pritchard
Nov 30, 2004·Trends in Molecular Medicine·Gee W LauFansheng Kong
Mar 24, 2005·Infection and Immunity·Nadine HoffmannNiels Høiby
Nov 2, 2005·FEMS Microbiology Letters·Egon A OzerJoseph Zabner
Oct 31, 2006·Biochemical and Biophysical Research Communications·Orit Rozenberg, Michael Aviram
Nov 24, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·David A StoltzDiana M Shih
Jun 15, 2007·Infection and Immunity·J Andy SchaberKendra P Rumbaugh
Dec 18, 2007·Cell·David E Clapham
Feb 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lynne R PrinceMoira K B Whyte
May 30, 2008·Clinical Reviews in Allergy & Immunology·Victoria E Wagner, Barbara H Iglewski
Aug 16, 2008·The Journal of Clinical Investigation·David A StoltzJoseph Zabner
Sep 20, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Balázs RadaThomas L Leto
Mar 17, 2009·Burns : Journal of the International Society for Burn Injuries·Michael MullerPeter K M Maitz

❮ Previous
Next ❯

Citations

Aug 27, 2011·Antioxidants & Redox Signaling·Zhi-Gang SheDe-Pei Liu
Nov 1, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yiorgos ApidianakisLaurence G Rahme
Jul 25, 2012·Lipids in Health and Disease·Ayman Samir Farid, Yoichiro Horii
Sep 20, 2012·Medical Microbiology and Immunology·Gisela von HovenMatthias Husmann
Oct 4, 2015·FEMS Microbiology Reviews·Catherine GrandclémentDenis D Faure
Dec 30, 2011·Current Opinion in Microbiology·Kendra P Rumbaugh, Gunnar F Kaufmann
Feb 25, 2015·Journal of Cancer Research and Clinical Oncology·Maximilian KrügerMaximilian Moergel
Oct 12, 2010·Atherosclerosis·Louis-Philippe PrécourtEmile Levy
Aug 7, 2013·Nanotoxicology·Nawel Ould-MoussaJean-François Berret
May 31, 2016·Chemico-biological Interactions·Clement E FurlongLucio G Costa
Apr 20, 2016·Infection and Immunity·Brahmchetna BediRuxana T Sadikot
May 27, 2010·IUBMB Life·Bharti MacknessMike Mackness
Jul 22, 2014·Journal of Basic Microbiology·Nalini EaswaranKodiveri Muthukaliannan Gothandam
Aug 17, 2019·Scientific Reports·Nicholas M MauriceRuxana T Sadikot
Jan 15, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Henning HagmannPaul Thomas Brinkkoetter
Jul 31, 2012·Cellular and Molecular Life Sciences : CMLS·Jos H M SchippersBernd Mueller-Roeber
Feb 11, 2021·Antioxidants·Giuseppe MancoTeresa Maria Carusone
Jul 4, 2021·Neurotoxicology and Teratology·Jacqueline M GarrickLucio G Costa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.