PMID: 11926994Apr 3, 2002Paper

Paraquat- and diquat-induced oxygen radical generation and lipid peroxidation in rat brain microsomes

Journal of Biochemistry
Kunio YuminoMasao Nakamura

Abstract

NADPH-menadione reductase activity by rat brain microsomes (Ms) was decreased 40-50% by 10 microM dicumarol, a potent inhibitor of DT-diaphorase, whereas no change in NADPH-paraquat (PQ) and -diquat (DQ) reductase activity was observed. NADPH-DQ reductase activity in brain Ms was 2.5-fold higher than NADPH-PQ reductase activity. The formation of PQ and DQ radicals was verified optically and observed directly by ESR spectroscopy in the NADPH-PQ and -DQ reductase reactions by brain Ms under anaerobic conditions. PQ- and DQ-induced superoxide formation was confirmed by the detection of DMPO-OOH ESR signals and followed by chemiluminescence (CL) of a Cypridina luciferin analogue (CLA). The kinetics and intensity of the CL were consistent with the observations that the reduction in DQ is faster than that in PQ. Thiobarbituric acid reactive substances (TBARS) and phospholipid hydroperoxides in brain Ms increased in the presence of NADPH and Fe3+. The generation of both lipid peroxidation products derived from brain Ms decreased with increasing concentrations of PQ and DQ. The inhibitory effect of DQ is more pronounced than that of PQ. The formation of PQ- and DQ-induced reactive oxygen species was not associated with lipid peroxidati...Continue Reading

Citations

Jul 8, 2011·Neurotoxicity Research·Li Rebekah Feng, Kathleen A Maguire-Zeiss
Mar 17, 2004·Trends in Pharmacological Sciences·Moreno PaoliniFrank J Gonzalez
Nov 28, 2012·The British Journal of Nutrition·Ping ZhengDaiwen Chen
Sep 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Won-Seok ChoiZhengui Xia
May 11, 2011·Antioxidants & Redox Signaling·Terina N Martinez, J Timothy Greenamyre
Jun 13, 2009·Proceedings. Biological Sciences·Ismael Galván, Carlos Alonso-Alvarez
Apr 5, 2011·Parkinson's Disease·P C KeaneC M Morris
Feb 15, 2012·Neurochemical Research·Senthilkumar S KaruppagounderMuralikrishanan Dhanasekaran
Nov 25, 2004·Critical Reviews in Toxicology·Shenggang LiJose de Leon
May 7, 2013·Progress in Neurobiology·Sudhakar Raja Subramaniam, Marie-Francoise Chesselet
Jul 13, 2014·Neurobiology of Disease·Sudhakar Raja SubramaniamMarie-Francoise Chesselet
Mar 29, 2011·Progress in Neurobiology·Hui-Ming Gao, Jau-Shyong Hong
Aug 4, 2007·Journal of the Neurological Sciences·Alexis Elbaz, Christine Tranchant
Apr 20, 2016·Free Radical Biology & Medicine·Raquel Requejo-Aguilar, Juan P Bolaños
May 8, 2015·Molecular Neurobiology·Chuen-Lin HuangNai-Kuei Huang
Jan 20, 2016·PloS One·Jia-Qing ZhangBao-Song Xing
May 14, 2005·Antioxidants & Redox Signaling·Xue-Fei WuJau-Shyong Hong
Dec 13, 2006·Biosensors & Bioelectronics·Jin Hyung LeeMan Bock Gu
Aug 22, 2008·Antioxidants & Redox Signaling·Douglas W Ethell, Qingyan Fei
May 14, 2005·Antioxidants & Redox Signaling·Serge Przedborski, Harry Ischiropoulos
Oct 17, 2007·Environmental Health Perspectives·Kavita PrasadEric K Richfield

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.