Parasitological and molecular diagnostic of a clinical Babesia caballi outbreak in Southern Romania

Parasitology Research
Mariana IonitaIoan Liviu Mitrea

Abstract

Equine piroplasmosis (EP) is a tick-borne disease of equids caused by Babesia caballi and/or Theileria equi, which is endemic in many tropical and temperate areas of the world. However, clinical outbreaks of EP in Romania during the last decades have not been reported Therefore, the aim of this paper is (i) to describe a clinical B. caballi outbreak in horses on several farms in Southern Romania using a diagnostic and therapeutic approach and (ii) the molecular diagnostic of EP in an endemic area of Romania. In the first case, a 10-month-old stallion male was presented with lethargy, anorexia, fever (40.9 °C), pale mucosal/mucous/membranes and a marked anemia. In the subsequent weeks, three horses from other farms located in the same area, displayed similar clinical signs. B. caballi was diagnosed in all the horses based on Giemsa-stained blood smears and the diagnosis was further confirmed by polymerase chain reaction (PCR), using a single-round and multiplex PCR and sequencing. All four horses were treated with imidocarb dipropionate, at a dose rate of 2.2 mg/kg body weight (two injections at 48 h apart), and all horses clinically recovered within 24-48 h, post-treatment. This report presents the first molecularly characteriz...Continue Reading

References

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Citations

Mar 7, 2021·Microorganisms·Andreea Monica BogdanIoan Liviu Mitrea

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Datasets Mentioned

BETA
MG760619
MG760622
EU642512.1
KR527220
KR527221
KJ908939
KJ908940
AY534883
AY309955
AY534883.1

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
PCR
light microscopy
electrophoresis

Software Mentioned

Basic Local Alignment Search Tool ( BLAST )
BLAST

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