Parathyroid hormone ameliorates temporomandibular joint osteoarthritic-like changes related to age

Cell Proliferation
Chen CuiXuedong Zhou

Abstract

Ageing could be a contributing factor to the progression of temporomandibular joint osteoarthritis (TMJ OA), whereas its pathogenesis and potential therapeutic strategy have not been comprehensively investigated. We generated ageing mouse models (45-week and 60-week; 12-week mice as control) and intermittently injected 45-week mice with parathyroid hormone (PTH(1-34)) or vehicle for 4 weeks. Cartilage and subchondral bone of TMJ were analysed by microCT, histological and immunostaining. Western blot, qRT-PCR, ChIP, ELISA and immunohistochemical analysis were utilized to examination the mechanism of PTH(1-34)'s function. We showed apparent OA-like phenotypes in ageing mice. PTH treatment could ameliorate the degenerative changes and improve bone microarchitecture in the subchondral bone by activating bone remodelling. Moreover, PTH inhibited phosphorylation level of Smad3, which can combine with p16ink4a gene promoter region, resulting in reduced senescent cells accumulation and increased cellular proliferation of marrow mesenchymal stem cells (MSCs). ELISA also showed relieved levels of specific senescent-associated secretory phenotype (SASP) in ageing mice after PTH treatment. In summary, PTH may reduce the accumulation of sen...Continue Reading

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Citations

Apr 4, 2021·International Journal of Molecular Sciences·Abhishek Chandra, Jyotika Rajawat

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Methods Mentioned

BETA
Assay
immunoprecipitation assay
PCRs
PCR
electrophoresis
Flow cytometry
ChIP

Software Mentioned

ImageJ
SPSS

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