PMID: 22338918Feb 22, 2012Paper

Parathyroid hormone enhances osteoblast differentiation from human skin derived precursor cells in vitro

Journal of the Medical Association of Thailand = Chotmaihet Thangphaet
Katesaree SuriyachandMonnipha Sila-Asna

Abstract

Parathyroid hormone (PTH), a new effective treatment for osteoporosis patients which promotes the anabolic effect in vivo, can enhance the differentiation of osteoblasts derived from the human skin-derived precursor cells (hSKPs) in vitro culture. This research investigated the effects of PTH by studying the gene expressions and other markers of osteoblast differentiation along with the induction of hSKPs to osteoblast in two experiment groups, i.e. the osteogenic induction medium (OM) only and the OM plus PTH (OM + PTH). The results of each type were compared between these two groups. Both groups expressed the Cbfa1 gene, a regulator of osteoblasts and also one of the most osteoblast specific genes. The findings were that the OM + PTH group showed more intense alkaline phosphatase staining than the other. The gene expressions of protein showing the mature osteoblasts like osteocalcin (OCN) and bone sialoprotein (BSP) in the OM + PTH group expressed higher and faster (Day 14) than the OM group. Moreover, the gene expression of osteoprotegerin (OPG) possessing the protein produced by the mature osteoblasts showed a higher level in the OM + PTH group on the same day as OCN and BSP occurred. This protein performs a function in inh...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.