PMID: 3761360Jan 1, 1986Paper

Parathyroid hormone-induced changes of the brush border topography and cytoskeleton in cultured renal proximal tubular cells

The Journal of Membrane Biology
M S GoligorskyK A Hruska

Abstract

In order to examine the possibility of parathyroid hormone-mediated ultrastructural rearrangements in target epithelium, isolated canine renal proximal tubular cells were grown on a collagen-coated semipermeable membrane in a defined medium. Scanning and transmission electron microscopy of these monolayers revealed abundant microvilli. Exposure of the proximal tubular cells to parathyroid hormone resulted in a biphasic changes involving: dramatic shortening and rarefaction of microvilli within 1 min; and recovery of microvillar topography after 5 min. A similar shortening of microvilli was observed following exposure to ionomycin, whereas incubation with cyclic AMP resulted in an elongation of microvilli. Parathyroid hormone stimulated cyclic AMP production and increased cytoplasmic free calcium concentration in cultured proximal tubular cells. Pretreatment of cells with a calmodulin inhibitor abolished the effect of parathyroid hormone on brush border topography. Shortening of microvilli was associated with a disappearance of microvillar core filaments. Staining of F-actin with fluoresceinphalloidin showed that parathyroid hormone resulted in fragmentation of stress fibers. It is concluded that parathyroid hormone-induced cell...Continue Reading

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Citations

Jun 24, 1987·Biochimica Et Biophysica Acta·C H van Os
Nov 1, 1988·Pflügers Archiv : European journal of physiology·J MerotP Poujeol
Jan 1, 1990·Archives of Toxicology·C PariatP Courtois
Jan 1, 1996·Cell Motility and the Cytoskeleton·J M SangerJ W Sanger
May 12, 1998·The American Journal of Physiology·Y ZhangA A McDonough
May 18, 1999·The American Journal of Physiology·Y ZhangA A McDonough
Oct 4, 2000·Physiological Reviews·H MurerJ Biber
Aug 1, 1987·The American Journal of Physiology·J A ColeL R Forte
Oct 1, 1989·The American Journal of Physiology·C B HensleyA K Mircheff
Sep 1, 1989·Kidney International·J Biber

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