Parathyroid hormone induces bone formation in phosphorylation-deficient PTHR1 knockin mice.

American Journal of Physiology. Endocrinology and Metabolism
Nabanita S DattaAbdul B Abou-Samra

Abstract

Activation of G protein-coupled receptors by agonists leads to receptor phosphorylation, internalization of ligand receptor complexes, and desensitization of hormonal response. The role of parathyroid hormone (PTH) receptor 1, PTHR1, is well characterized and known to regulate cellular responsiveness in vitro. However, the role of PTHR1 phosphorylation in bone formation is yet to be investigated. We have previously demonstrated that impaired internalization and sustained cAMP stimulation of phosphorylation-deficient (PD) PTHR1 leads to exaggerated cAMP response to subcutaneous PTH infusion in a PD knockin mouse model. To understand the physiological role of receptor internalization on PTH bone anabolic action, we examined bone parameters of wild-type (WT) and PD knockin female and male mice following PTH treatment. We found a decrease in total and diaphyseal bone mineral density in female but not in male PD mice compared with WT controls at 3-6 mo of age. This effect was attenuated at older age groups. PTH administration displayed increased bone volume and trabecular thickness in the vertebrae and distal femora of both WT and PD animals. These results suggest that PTHR1 phosphorylation does not play a major role in the anabolic...Continue Reading

References

Jan 1, 1992·Cold Spring Harbor Symposia on Quantitative Biology·R J LefkowitzM G Caron
Nov 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M E MarshR T Franceschi
May 15, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Akiko Iida-KleinRobert Lindsay
Aug 16, 2003·The Journal of Biological Chemistry·W Bruce SneddonPeter A Friedman
Mar 15, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Mary L BouxseinSerge L Ferrari
May 11, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Nabanita S DattaLaurie K McCauley
Jan 18, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Robert LindsayAnthony B Hodsman
Feb 20, 2007·Annual Review of Physiology·Scott M DeWireSudha K Shenoy
May 16, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Nabanita S DattaLaurie K McCauley
Mar 3, 2009·Cellular Signalling·Nabanita S Datta, Abdul B Abou-Samra
Aug 25, 2009·Nature Chemical Biology·Sébastien FerrandonJean-Pierre Vilardaga
Mar 10, 2010·The Journal of Biological Chemistry·Guillermo RomeroPeter A Friedman
Aug 13, 2010·Cellular and Molecular Life Sciences : CMLS·Jean-Pierre VilardagaThomas J Gardella
Oct 12, 2010·The Journal of Endocrinology·Nabanita S DattaAbdul B Abou-Samra

❮ Previous
Next ❯

Citations

Oct 19, 2013·Journal of Mammary Gland Biology and Neoplasia·Aaron M MarshallNelson D Horseman
Mar 7, 2021·Journal of Cellular and Molecular Medicine·Chaonan Sun, Shenglong Li
Jun 9, 2021·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Laura E ZweiflerLaurie K McCauley

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.