Parathyroid hormone induces prostaglandin G/H synthase-2 expression by a cyclic adenosine 3',5'-monophosphate-mediated pathway in the murine osteoblastic cell line MC3T3-E1

Endocrinology
S TetradisB E Kream

Abstract

Prostaglandin G/H synthase (PGHS), a central enzyme for PG synthesis, is encoded by the constitutively expressed PGHS-1 and the inducible PGHS-2. The goal of this project was to study the regulation of PGHS-2 gene expression by PTH and its possible signaling pathways in osteoblastic MC3T3-E1 cultures. Bovine PTH-(1-34) at 0.01-10 nM increased PGHS-2, but not PGHS-1, messenger RNA (mRNA) levels. The effect of PTH was maximal at 1 h and decreased almost to control levels by 6 h. Phorbol myristate acetate (PMA), forskolin, and 8-bromo-cAMP increased PGHS-2 mRNA levels, whereas ionomycin had no effect. PTH, forskolin, and PMA increased the release of PGE2 into the culture medium. Pretreatment of cells with 0.1 microM PMA for 16 h blocked the induction of PGHS-2 mRNA levels by PMA, but did not alter the effects of PTH and forskolin. However, treatment of cells with 30 microM H-89, a protein kinase A inhibitor, significantly reduced the ability of PTH and forskolin to induce PGHS-2 mRNA levels. Moreover, PTH-(3-34) at 0.1-100 nM did not induce PGHS-2 mRNA levels. Our results show that PTH can rapidly and transiently induce PGHS-2 mRNA levels in osteoblastic MC3T3-E1 cells, primarily via the cAMP-protein kinase A signal transduction p...Continue Reading

Citations

Dec 1, 1999·British Journal of Pharmacology·J A Mitchell, T D Warner
Dec 9, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S TetradisB E Kream
Oct 9, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·W WindischhoferH J Leis
Jun 17, 2004·Current Opinion in Nephrology and Hypertension·Nancy S KriegerDavid A Bushinsky
Jul 14, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Y K MinC C Pilbeam
Nov 25, 2003·Seminars in Dialysis·Nancy S KriegerKevin K Frick
May 30, 2006·Bone·Galen RobertsonXinping Zhang
Oct 20, 2007·Biochimica Et Biophysica Acta·Thomas KleinMartin Kömhoff
Aug 15, 2003·Immunological Investigations·Cheorl-Ho KimYoung-Guk Park
Nov 13, 2001·American Journal of Physiology. Renal Physiology·D A BushinskyN S Krieger
Nov 30, 2000·American Journal of Physiology. Renal Physiology·N S KriegerD A Bushinsky

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