PMID: 9556130Apr 29, 1998Paper

Parathyroid hormone regulates the expression of the nuclear mitotic apparatus protein in the osteoblast-like cells, ROS 17/2.8

Bone
K TorrungruangJoseph P Bidwell

Abstract

The parathyroid hormone (PTH) signaling pathways that effect changes in osteoblast gene expression also alter the organization of the cytoskeletal proteins. PTH regulates the expression of nucleoskeletal proteins, such as nuclear mitotic apparatus protein (NuMA) and topoisomerase II-alpha. NuMA is a structural component of the interphase nucleus and organizes the microtubules of the mitotic spindle during mitogenesis. We propose that PTH-induced alterations in osteoblast cytoarchitecture are accompanied by changes in osteoblast nuclear structure that contribute to changes in gene expression. We used immunofluorescence and confocal microscopy to determine the effect of PTH on the expression and nuclear distribution of NuMA in the rat osteosarcoma cell line, ROS 17/2.8. Cells were treated with PTH or vehicle, then fixed and stained with NuMA antibody. Optical sections of interphase naive cells revealed a diffuse distribution of NuMA, interspersed with speckles, in the central nuclear planes but not in nucleoli. During the metaphase and anaphase, NuMA localized at the mitotic spindle apparatus. The percentage of NuMA-immunopositive ROS 17/2.8 cells decreased with increasing confluence, but serum starvation did not attenuate NuMA e...Continue Reading

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Citations

Jun 8, 2000·Microscopy Research and Technique·C Zeng
Sep 6, 2000·Journal of Cellular Biochemistry·H A FeisterJ P Bidwell
Mar 12, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J P BidwellJ Hock
Jun 7, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·P H WatsonA B Hodsman
Nov 13, 2012·Critical Reviews in Eukaryotic Gene Expression·Joseph P BidwellFeng-Chun Yang
Nov 4, 2000·IEEE Transactions on Bio-medical Engineering·S K YooM W Vannier
Jul 22, 2004·Experimental Cell Research·Pekka TaimenMarkku Kallajoki
Mar 31, 2000·Experimental Cell Research·P TaimenM Kallajoki
Sep 22, 2009·Bone·Paul ChildressJoseph P Bidwell

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