PMID: 9186276May 1, 1997Paper

Parathyroid hormone-related protein in neonatal and reproductive goats determined by a sensitive time-resolved immunofluorometric assay

European Journal of Endocrinology
H RongE Bucht

Abstract

High concentrations of parathyroid hormone-related protein (PTHrP) have been found in goat milk but it is not known whether it can enter the circulation of the neonate. In this study we have developed a sensitive two-site lanthanide immunofluorometric assay (IFMA) using dissociation and enhancement time-resolved fluorometry to address this question. Affinity-purified anti-PTHrP 38-67 raised in rabbit was biotinylated and immobilized in streptavidin-coated microtitration wells as a 'capture' antibody. As a signal, affinity-purified anti-PTHrP 1-34, raised in sheep, was labeled with an europium chelate. A sensitivity of 0.3 pmol/l was achieved. PTHrP levels were determined in the plasma of eleven neonatal, seven parturient and six non-pregnant, non-lactating goats as well as in goat milk. The circulating PTHrP levels (mean +/- S.D.) were significantly increased at day 1 (6.1 +/- 1.7 pmol/l: P < 0.01) and day 3 (3.5 +/- 0.6 pmol/l: P < 0.05) after birth in the male kids (n = 8) bottle-fed with milk from the dams, compared with before (2.2 +/- 0.7 pmol/l) and 30 min after (2.0 +/- 0.6 pmol/l) the first feeding and 14 days (2.4 +/- 0.8 pmol/l) later. In the female kids (n = 3) fed with formula there was no such increase and the conc...Continue Reading

Citations

Nov 5, 2003·The Journal of Clinical Investigation·Joshua N VanHoutenJohn J Wysolmerski
Jun 3, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R JemtlandK M Gautvik
Dec 27, 2005·FEBS Letters·Adelino V M CanarioMelody S Clark
Dec 31, 1997·Endocrine Reviews·C S Kovacs, H M Kronenberg
Jun 15, 2006·American Journal of Physiology. Endocrinology and Metabolism·Deenaz ZaidiGraham F Wagner
Nov 5, 1999·Biochemical and Biophysical Research Communications·H RongE Bucht

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