Parathyroid hormone stimulates fra-2 expression in osteoblastic cells in vitro and in vivo

Endocrinology
Laurie K McCauleyL R McCabe

Abstract

PTH and PTH-related protein (PTHrP) are key mediators of skeletal development and homeostasis through their activation of the PTH-1 receptor. Previous studies have found that several AP-1 family members are regulated by PTH, such as c-fos, fra-1, and c-jun. There are numerous genes in the bone microenvironment that contain AP-1 sites, and different Fos family members are reported to have opposing transcriptional activities at AP-1 sites. The purpose of this study was to identify the effects of PTH on expression of the AP-1 protein complex member, fra-2, to extend our understanding of transcriptional regulators of PTH action. PTH induction of fra-2 messenger RNA (mRNA) levels in MC3T3-E1 preosteoblastic cells was maximal with 0.1 microM PTH (1-34). The expression in vitro was greatest 1 h after treatment and was present with N-terminal PTH but not PTH (7-34) or (53-84). Cycloheximide treatment induced fra-2 expression, and actinomycin D inhibited basal and PTHrP-induced expression. AP-1 protein in nuclear extracts of MC3T3-E1 cells was increased with PTH treatment at 3 h and consisted of high levels of Fra-2 protein, as evidenced by a supershift in an electrophoretic mobility shift assay and Western blot analysis. Up-regulation ...Continue Reading

References

Dec 1, 1992·Molecular and Cellular Endocrinology·J M Ness, B G Kasson
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·T Hai, T Curran
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H NishinaH Iba
Nov 4, 1988·Cell·T Curran, B R Franza
Jun 11, 1993·Nucleic Acids Research·T YoshidaH Iba
Jul 1, 1994·Journal of Cellular Biochemistry·N C PartridgeA T Pearman
Nov 1, 1995·Trends in Genetics : TIG·A E GrigoriadisE F Wagner
Aug 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·L K McCauleyR T Franceschi
Apr 1, 1996·Physiological Reviews·G S SteinM Montecino
Apr 1, 1996·Immunology and Cell Biology·V C Foletta
Jan 20, 2000·The New England Journal of Medicine·G J Strewler
Mar 15, 2000·Molecular and Cellular Endocrinology·J RubinW Hofstetter

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Citations

Apr 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ryo HanyuMasaki Noda
Jul 5, 2007·Histochemistry and Cell Biology·Lindsay M Martin, Laura R McCabe
May 11, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Nabanita S DattaLaurie K McCauley
May 16, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Nabanita S DattaLaurie K McCauley
Jan 19, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Janice E BerryLaurie K McCauley
Apr 24, 2013·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Changjun LiMei Wan
Dec 25, 2009·Journal of Tissue Engineering and Regenerative Medicine·Ramakrishnaiah SiddappaJan de Boer
Aug 29, 2014·Journal of Cellular Biochemistry·Shuichi MoriyaMasaki Noda
Feb 2, 2008·Journal of Cellular Biochemistry·Aya KawamataMasaki Noda
Oct 18, 2005·Journal of Cellular Biochemistry·Majd Zayzafoon
Sep 20, 2002·Endocrinology·Burak DemiralpLaurie K McCauley
Oct 16, 2004·The Journal of Biological Chemistry·Flavia Q PirihSotirios Tetradis
Aug 10, 2004·The Journal of Biological Chemistry·Weizhong ChangThomas L McCarthy

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