PMID: 9178105Jun 15, 1997Paper

Parathyroid hormone uses both adenylate cyclase and protein kinase C to regulate acid production in osteoclasts

Journal of Cellular Biochemistry
L G May, C V Gay

Abstract

Osteoclasts, isolated from the endosteum of 2.5- to 3-week-old chickens, were treated with acridine orange, a hydrogen ion concentration-sensitive fluorescent dye, in order to monitor changes in acid production. The adenylate cyclase inhibitor, alloxan, blocked parathyroid hormone (PTH)-stimulated acid production. Dibutyryl cyclic adenosine monophosphate, a membrane-permeant form of cyclic adenosine monophosphate, mimicked the PTH effect. Bisindolylmaleimide, a specific inhibitor of protein kinase C (PKC), blocked the initial stimulation (15, 30, and 60 min) of acid production by PTH but had no effect on long-term stimulation (120 min). Confocal microscopy of osteoclasts stained with fluorescein-conjugated bisindolylmateimide revealed a shift in location of PKC from the cytoplasm to the plasma membrane region after treatment with parathyroid hormone. The results of these studies support the hypothesis that PTH regulation of acid production in osteoclasts involves both adenylate cyclase and PKC as effectors.

References

May 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·N Agarwala, C V Gay
Oct 1, 1992·Trends in Biochemical Sciences·J R Hepler, A G Gilman
Jan 1, 1992·Endocrinology·H JouishommeR H Rixon
Feb 14, 1991·Biochemical and Biophysical Research Communications·A TetiA Zambonin Zallone
Jan 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S J HunterC V Gay
Jun 1, 1988·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S J HunterC V Gay
Dec 1, 1985·Calcified Tissue International·R E AndersonD M Woodbury
May 16, 1984·Biochemical and Biophysical Research Communications·A Rodriguez-Pena, E Rozengurt
Jan 1, 1983·Metabolic Bone Disease & Related Research·C V GayH Schraer
Jul 1, 1994·Journal of Cellular Biochemistry·N C PartridgeA T Pearman
Apr 29, 1994·Biochemical and Biophysical Research Communications·K D Brubaker, C V Gay
May 14, 1993·Biochemical and Biophysical Research Communications·C V GayP J Bekker
Aug 1, 1993·The Journal of Surgical Research·J Sack
May 1, 1996·In Vitro Cellular & Developmental Biology. Animal·L G May, C V Gay

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Citations

Nov 26, 2009·Proceedings of the National Academy of Sciences of the United States of America·Wei MaGerald A Berkowitz
Mar 30, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M C LangubH H Malluche
Jul 23, 2003·Journal of Cellular Biochemistry·Carol V GayVirginia R Gilman
Jun 8, 2001·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·K.-D. Schlüter

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