PMID: 9192514Jul 1, 1997Paper

Parathyroid hormone versus phorbol ester stimulation of activator protein-1 gene family members in rat osteosarcoma cells

Calcified Tissue International
R C KoeN C Partridge

Abstract

We have previously shown that in the rat osteoblastic osteosarcoma cell line-UMR 106-01-PTH induces maximal collagenase mRNA levels at 4 hours. Since this response to PTH requires de novo protein synthesis, it may be mediated by the combined temporal expression of members of the activator protein-1 (AP-1) gene family. We have demonstrated that maximal mRNA levels of two of the members of this family, c-fos and c-jun, occur 30 min after stimulation by PTH. Phorbol myristate acetate (PMA) elicits a similar increase in c-fos and c-jun mRNAs, but is unable to stimulate transcription of collagenase in these cells. To investigate further the involvement of the AP-1 gene family, we examined PTH and PMA stimulation of jun-B, jun-D, fos B, and fra-1 mRNAs in UMR 106-01 cells. The mRNA for jun-D was abundant under control conditions and showed no variation in response to PTH (10(-8) M). The fos B transcripts were not detected under control conditions, whereas jun-B and fra-1 mRNAs were present at low basal levels. PTH caused an increase in fos B mRNA that reached a maximal 4- to 5-fold plateau between 45 and 60 min. An increase in jun-B mRNA in response to PTH was detectable at 30 min, but reached a maximal 6- to 7-fold increase at 2 hou...Continue Reading

Citations

Sep 1, 2000·Journal of Cellular Biochemistry·N SelvamuruganN C Partridge
Sep 1, 2000·Journal of Cellular Biochemistry·M ZayzafoonL R McCabe
May 30, 2001·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·J R GardnerV Gulani
Jan 30, 2002·Gene·John T SwarthoutNicola C Partridge
May 10, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Y Wu, R Kumar
Mar 3, 2009·Cellular Signalling·Nabanita S Datta, Abdul B Abou-Samra
Mar 24, 2009·Journal of Cellular Biochemistry·Supaporn SuttamanatwongRajaram Gopalakrishnan
Jan 19, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Janice E BerryLaurie K McCauley
Dec 25, 2009·Journal of Tissue Engineering and Regenerative Medicine·Ramakrishnaiah SiddappaJan de Boer
Aug 5, 2014·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Leila RevolloRoberto Civitelli
Jul 13, 2000·Molecular Cell Biology Research Communications : MCBRC·N Selvamurugan, N C Partridge
Feb 8, 2002·American Journal of Physiology. Endocrinology and Metabolism·Mary S Erclik, Jane Mitchell

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