Paraxial charge compensator for electron cryomicroscopy.

Ultramicroscopy
John A Berriman, Peter B Rosenthal

Abstract

We describe a multi-hole condenser aperture for the production of several electron beams in the transmission electron microscope (TEM) making it possible to simultaneously image and irradiate spatially separated regions of a specimen. When the specimen is a thin film of vitreous ice suspended over a holey carbon film, simultaneous irradiation of the adjacent carbon support with the off-axis beam compensates for some of the effects of charging in the image formed by a beam irradiating only the ice. Because the intervening region is not irradiated, charge-neutralization of frozen-hydrated specimens can occur by a through-space mechanism such as the emission of secondary electrons from a grounded carbon support film. We use paraxial charge compensation (PCC) to control the amount of charge build-up on the specimen and observe the effects of charge on images. The multi-hole aperture thus provides a tool for investigating the mechanism of charging and charge mitigation during the imaging of radiation sensitive biological specimens by cryomicroscopy.

References

Jan 1, 1979·Ultramicroscopy·S B Hayward, R M Glaeser
May 25, 1975·Journal of Molecular Biology·P N Unwin, R Henderson
May 27, 1971·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·P N Unwin
Jan 1, 1996·Journal of Structural Biology·R A CrowtherJ M Smith
Jan 1, 1996·Journal of Structural Biology·P A Thuman-Commike, W Chiu
Apr 22, 2004·Journal of Structural Biology·Nobuhiko GyobuYoshinori Fujiyoshi
May 4, 2004·Micron : the International Research and Review Journal for Microscopy·R F EgertonM Malac
Aug 13, 2004·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Dieter TypkeRobert M Glaeser
Aug 16, 2006·Journal of Structural Biology·Matthew L BakerChandrajit Bajaj
Jun 23, 2009·Science·A K Geim
Dec 1, 2004·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Kenneth H DowningRobert M Glaeser
Dec 1, 2004·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Robert M Glaeser, Kenneth H Downing
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·John A BerrimanPeter B Rosenthal
Jan 20, 2010·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Craig YoshiokaClinton S Potter
May 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Lesley J CalderPeter B Rosenthal
Sep 28, 2010·Nucleic Acids Research·Nicole M BakerAlfonso Mondragón

❮ Previous
Next ❯

Citations

Jan 18, 2016·Current Opinion in Structural Biology·Christopher J Russo, Lori A Passmore
Nov 26, 2015·Journal of Structural Biology·Christopher J Russo, Lori A Passmore
Feb 13, 2016·IUCrJ·Sriram SubramaniamRichard Henderson
Dec 30, 2014·Trends in Biochemical Sciences·Xiao-chen BaiSjors H W Scheres
Sep 24, 2016·Quarterly Reviews of Biophysics·Kutti R Vinothkumar, Richard Henderson
Jul 10, 2018·Angewandte Chemie·Richard Henderson
Sep 4, 2018·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Rebecca S DillardElizabeth R Wright
Dec 12, 2020·Journal of Structural Biology·Natàlia de Martín GarridoChristopher H S Aylett

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Related Papers

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
Robert M Glaeser, K H Downing
Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
K H DowningRobert M Glaeser
Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
Michael B Sherman, Wah Chiu
© 2021 Meta ULC. All rights reserved