Parental LTRs are important in a construct of a stable and efficient replication-competent infectious molecular clone of HIV-1 CRF08_BC.

PloS One
Qiwei ZhangBo-Jian Zheng

Abstract

Circulating recombinant forms (CRFs) of HIV-1 have been identified in southern China in recent years. CRF08_BC is one of the most predominant subtypes circulating in China. In order to study HIV subtype biology and to provide a tool for biotechnological applications, the first full-length replication-competent infectious molecular clone harboring CRF08_BC is reported. The construction of this clone pBRGX indicates that a moderate-copy number vector is required for its amplification in E. coli. In addition, it is shown that the parental CRF08_BC LTRs are important for generating this efficient replication-competent infectious clone. These observations may aid in the construction of infectious clones from other subtypes. Both the pBRGX-derived virus and its parental isolate contain CCR5 tropism. Their full-length genomes were also sequenced, analyzed, compared and deposited in GenBank (JF719819 and JF719818, respectively). The availability of pBRGX as the first replication-competent molecular clone of CRF08_BC provides a useful tool for a wide range of studies of this newly emergent HIV subtype, including the development of HIV vaccine candidates, antiviral drug screening and drug resistance analysis.

References

Sep 30, 1991·Biochemical and Biophysical Research Communications·K PrakashS A Plotkin
Nov 16, 1990·Cell·R A MarciniakP A Sharp
Oct 3, 1999·AIDS Research and Human Retroviruses·M H NaghaviA Vahlne
Oct 3, 1999·AIDS Research and Human Retroviruses·N MochizukiM Tatsumi
Jan 19, 2000·Nucleic Acids Research·L A PereiraN J Deacon
Jan 24, 2002·AIDS Research and Human Retroviruses·P NovelliR S Daniels
Oct 31, 2002·AIDS Research and Human Retroviruses·Hiroaki TakeuchiYoshio Koyanagi
Jul 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Koichiro TamuraSudhir Kumar
Mar 29, 2005·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Xiu-Ying ZhaoBo-Jian Zheng
Jun 1, 2005·Cellular and Molecular Life Sciences : CMLS·Gilles Courtois
Feb 9, 2008·AIDS Research and Human Retroviruses·Zhefeng MengYiming Shao
Apr 17, 2010·AIDS·William H WheelerUNKNOWN Variant, Atypical, and Resistant HIV Surveillance Group
Oct 26, 2010·AIDS Research and Human Retroviruses·María L RauddiSilvia A González

❮ Previous
Next ❯

Citations

Dec 9, 2014·AIDS Research and Human Retroviruses·Hao WuBo-Jian Zheng

❮ Previous
Next ❯

Datasets Mentioned

BETA
JF719819

Methods Mentioned

BETA
PCR
ELISA
transfection
Staining

Software Mentioned

CLUSTAL
SimPlot
BootScan
BLAST
MEGA
Seqman Pro

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.