Parentally inherited long non-coding RNA Cyrano is involved in zebrafish neurodevelopment

Nucleic Acids Research
Mayuresh Anant SarangdharBeena Pillai

Abstract

Transfer of genetic material from parents to progeny via fusion of gametes is a way to ensure flow of information from one generation to the next. Apart from the genetic material, gametes provide a rich source of other factors such as RNA and proteins which can control traits of the embryo. Non-coding RNAs are not only carriers of regulatory information but can also encode memory of events of parental life. Here, we explore the possibility of parental inheritance of non-coding RNAs, especially long non-coding RNAs. Meta-analysis of RNA-seq data revealed several non-coding RNAs present in zebrafish oocyte, sperm and 2cell-stage. The embryo is transcriptionally silent at this stage, we rationalize that all the RNAs detectable at 2cell-stage are deposited either by sperm or oocyte or both and thus inherited. In the inherited pool, we noticed a conserved lncRNA, Cyrano previously known for zebrafish brain development. Knockdown of inherited Cyrano by miR-7 without changing zygotic Cyrano altered brain morphology at 24 hpf and 48 hpf. This defect could be partially rescued by injecting full length Cyrano lncRNA or a mutant resilient to knock-down by miR-7. In future, there is ample scope to check the possibility of inherited lncRNAs...Continue Reading

References

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Citations

May 3, 2019·RNA·Perrine LavalouAlena Shkumatava
Aug 3, 2019·Scientific Reports·Marta F RiescoVanesa Robles
Aug 21, 2019·Gene·Satheeswaran BalasubramanianEkambaram Perumal
Aug 25, 2020·The Canadian Journal of Cardiology·Andrew I LumleyYvan Devaux
Jan 8, 2021·Mobile DNA·Ema EtchegarayZofia Haftek-Terreau
Mar 24, 2021·Briefings in Functional Genomics·Gyan RanjanSridhar Sivasubbu
May 30, 2021·Translational Stroke Research·Weijie ChenPeiying Li
May 18, 2021·Frontiers in Molecular Neuroscience·Norjin ZolbootGiordano Lippi

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Methods Mentioned

BETA
RNA-seq
PCR
transgenic

Software Mentioned

BLAST
Cuffquant
ImageJ
Cyrano
FastQC
Tophat
Ensembl
Trimmomatic

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