Parenteral nutrition rapidly reduces hepatic mononuclear cell numbers and lipopolysaccharide receptor expression on Kupffer cells in mice

JPEN. Journal of Parenteral and Enteral Nutrition
Jiro OmataKazuo Hase

Abstract

Parenteral nutrition (PN) reduces the number of hepatic mononuclear cell (MNCs) and impairs their function, resulting in poor survival after intraportal bacterial challenge in mice. Our recent animal study demonstrated resumption of enteral nutrition after PN to rapidly restore hepatic MNC numbers (in 12 hours) and lipopolysaccharide (LPS) receptor expression on Kupffer cells (in 48 hours). The present study examined the time courses of hepatic MNC number reductions and LPS receptor expression changes in mice receiving PN. Male mice (n = 49) from the Institute of Cancer Research were divided into chow (n = 8), PN0.5 (n = 8), PN1 (n = 8), PN2 (n = 9), PN3 (n = 9), and PN5 (n = 7) groups. The chow group was given chow with an intravenous saline infusion. The PN groups were fed parenterally for 0.5, 1, 2, 3, or 5 days following the chow-feeding courses. After 7 days of nutrition support, hepatic MNCs were isolated and counted. The expression of LPS receptors on Kupffer cells was analyzed by flow cytometry. Hepatic MNC numbers rapidly reached their lowest level in the PN0.5 and PN1 groups but were somewhat restored thereafter and remained stable after the third day, without significant differences between any 2 of the PN groups. CD...Continue Reading

References

Jul 1, 1992·The American Journal of Physiology·T A FralixE Murphy
Nov 1, 1983·JPEN. Journal of Parenteral and Enteral Nutrition·H S SitrenJ J Cerda
Nov 1, 1994·Shock·L BortenschlagerG P Zaloga
Jun 1, 1996·Annals of Surgery·K A KudskK B Renegar
May 12, 2000·Immunological Reviews·P A Knolle, G Gerken
Jul 19, 2002·The New England Journal of Medicine·Stefan KiechlDavid A Schwartz
Apr 5, 2005·Toxicologic Pathology·George A Parker, Catherine A Picut
Apr 5, 2007·Annals of the New York Academy of Sciences·Herbert Bosshart, Michael Heinzelmann
Nov 6, 2007·Seminars in Liver Disease·Bertus EksteenDavid H Adams
Jan 22, 2008·Gut·D H AdamsS M Curbishley
Feb 19, 2008·Shock·Hironori TsujimotoHidetaka Mochizuki
Jun 23, 2009·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Jiro OmataKazuo Hase

❮ Previous
Next ❯

Citations

Jan 31, 2016·Journal of Pediatric Gastroenterology and Nutrition·Iva HojsakUNKNOWN ESPGHAN Committee on Nutrition

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry

Software Mentioned

StatView

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.