Nov 8, 2018

PARIS, an optogenetic method for functionally mapping gap junctions

BioRxiv : the Preprint Server for Biology
Ling WuYulong Li

Abstract

Cell-cell communication via gap junctions regulates a wide range of physiological processes by enabling the direct intercellular electrical and chemical coupling. However, the in vivo distribution and function of gap junctions remain poorly understood, partly due to the lack of non-invasive tools with both cell-type specificity and high spatiotemporal resolution. Here we developed PARIS (pairing actuators and receivers to optically isolate gap junctions), a new fully genetically encoded tool for measuring the cell-specific gap junctional coupling (GJC). PARIS successfully enabled monitoring of GJC in several cultured cell lines under physiologically relevant conditions and in distinct genetically defined neurons in Drosophila brain, with ~10-sec temporal resolution and sub-cellular spatial resolution. These results demonstrate that PARIS is a robust, highly sensitive tool for mapping functional gap junctions and study their regulation in both health and disease.

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Mentioned in this Paper

In Vivo
Regulation of Biological Process
Neurons
Spatial Distribution
Extracellular Space
Brain
Drosophila
Cell Communication
Isolate compound
Gap Junction Activity

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