PMID: 568773Sep 6, 1978

Parotid acinar cells: ionic dependence of acetylcholine-evoked membrane potential changes

Pflügers Archiv : European journal of physiology
M L RobertsO H Petersen

Abstract

Segments of mouse parotid were placed in a superfusion chamber. Surface acini were impaled by one or two micro-electrodes for measurement of membrane potential and resistance. The acinus under investigation was stimulated by micro-iontophoretic application of acetylcholine (ACh) or adrenaline. Neighbouring acinar cells were electrically coupled. Electrical coupling between acinar cells only occurred within restricted domains probably corresponding to an acinus or a group of acini. Passing direct current through one intracellular electrode, the resting potential of an acinus could be set at desired levels and the dependency of the ACh-evoked potential change on the resting potential investigated. The ACh null potential (initial effect) was about--60 mV. A delayed hyperpolarizing effect of ACh could not be reversed. The initial ACh-evoked potential change was sensitive to alterations in extracellular Na, K and Cl concentration. The delayed ACh-evoked hyperpolarization was blocked by ouabain, exposure to Na-free or K-free solutions. It is concluded that ACh increases mainly K and Na membrane conductance causing K efflux and Na influx with a subsequent Na activation of an electrogenic Na pump.

References

Jul 29, 1977·Pflügers Archiv : European journal of physiology·H R KoelzI Schulz
Jan 1, 1977·Pflügers Archiv : European journal of physiology·O H PetersenT A Gray
Jan 1, 1978·The Journal of Physiology·N Iwatsuki, O H Petersen
Jan 1, 1976·The Journal of Physiology·O H Petersen, N Ueda
Aug 1, 1977·The Journal of Physiology·N Iwatsuki, O H Petersen
Aug 31, 1973·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·O H Petersen
Nov 28, 1973·Biochimica Et Biophysica Acta·B L Ginsborg
Oct 1, 1973·The Journal of Physiology·G L Pedersen, O H Petersen
Feb 1, 1974·The Journal of Physiology·B L GinsborgE M Silinsky
Jan 1, 1974·The Journal of Membrane Biology·O H Petersen, G L Pedersen
Apr 1, 1974·The Journal of Physiology·A Nishiyama, O H Petersen
Jul 9, 1974·Pflügers Archiv : European journal of physiology·J H Poulsen
Sep 17, 1957·Acta Physiologica Scandinavica·A LUNDBERG
Jan 1, 1958·Pflügers Archiv für die gesamte Physiologie des Menschen und der Tiere·W TRAUTWEIN, J DUDEL
Oct 1, 1960·The American Journal of Physiology·L H SCHNEYER, C A SCHNEYER

Citations

Sep 1, 1990·Pflügers Archiv : European journal of physiology·S DissingO Sten-Knudsen
Jan 1, 1981·Pflügers Archiv : European journal of physiology·D V Gallacher, O H Petersen
Jul 1, 1979·Pflügers Archiv : European journal of physiology·N Iwatsuki, O H Petersen
Apr 1, 1982·Pflügers Archiv : European journal of physiology·N Iwatsuki, A Nishiyama
Aug 1, 1980·Pflügers Archiv : European journal of physiology·M Wakui, A Nishiyama
Jan 1, 1984·Pflügers Archiv : European journal of physiology·G T Pearson, O H Petersen
Sep 17, 1979·Life Sciences·B A LeslieJ W Putney
Jan 1, 1988·Comparative Biochemistry and Physiology. A, Comparative Physiology·B Nauntofte, S Dissing
Mar 1, 1981·Acta Physiologica Scandinavica·P Thesleff
Jan 1, 1980·Annals of the New York Academy of Sciences·J A YoungI Novak
Nov 13, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ji Eun LeeSung Joon Kim
Oct 29, 1981·Nature·O H Petersen
Jan 24, 1980·Nature·D V Gallacher, O H Petersen
Aug 1, 1985·The Anatomical Record·M BjerknesS Erlandsen
Jan 1, 1990·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J R Martinez
Oct 1, 1981·Scottish Medical Journal·S T Green

Related Concepts

Acetylcholine Sulfate (1: 1)
Chloride Ion Level
Resting Potentials
Osmolality
Ouabain
Parotid Gland
Potassium
Sodium

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.