PARP1-dependent eviction of the linker histone H1 mediates immediate early gene expression during neuronal activation

The Journal of Cell Biology
Gajendra Kumar AzadEran Meshorer

Abstract

Neuronal stimulation leads to immediate early gene (IEG) expression through calcium-dependent mechanisms. In recent years, considerable attention has been devoted to the transcriptional responses after neuronal stimulation, but relatively little is known about the changes in chromatin dynamics that follow neuronal activation. Here, we use fluorescence recovery after photobleaching, biochemical fractionations, and chromatin immunoprecipitation to show that KCl-induced depolarization in primary cultured cortical neurons causes a rapid release of the linker histone H1 from chromatin, concomitant with IEG expression. H1 release is repressed by PARP inhibition, PARP1 deletion, a non-PARylatable H1, as well as phosphorylation inhibitions and a nonphosphorylatable H1, leading to hindered IEG expression. Further, H1 is replaced by PARP1 on IEG promoters after neuronal stimulation, and PARP inhibition blocks this reciprocal binding response. Our results demonstrate the relationship between neuronal excitation and chromatin plasticity by identifying the roles of polyadenosine diphosphate ribosylation and phosphorylation of H1 in regulating H1 chromatin eviction and IEG expression in stimulated neurons.

References

Dec 4, 2002·Nature Reviews. Neuroscience·Anne E WestMichael E Greenberg
May 3, 2003·The EMBO Journal·Josiane Ménissier de MurciaGilbert de Murcia
Jun 18, 2003·Cell·W Lee Kraus, John T Lis
Nov 11, 2003·Genes, Brain, and Behavior·J L R Rubenstein, M M Merzenich
Nov 13, 2003·Molecular and Cellular Biology·Alejandro ContrerasRafael E Herrera
Feb 14, 2004·Journal of Cell Science·Michèle RouleauGuy G Poirier
Jun 19, 2004·Science·Malka Cohen-ArmonJames H Schwartz
Aug 24, 2005·Proceedings of the National Academy of Sciences of the United States of America·Vardhan S DaniSacha B Nelson
Jan 10, 2006·Developmental Cell·Eran MeshorerTom Misteli
Sep 2, 2006·The EMBO Journal·José YélamosJosiane Ménissier-de Murcia
Mar 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ignacio E SchorAlberto R Kornblihtt
Jul 31, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·A Iván HernándezSamuel Schacher
Aug 4, 2009·Journal of Neurochemistry·Shmuel GoldbergMalka Cohen-Armon
Oct 3, 2009·Nature Protocols·Nicolas GaspardPierre Vanderhaeghen
Apr 16, 2010·Nature·Tae-Kyung KimMichael E Greenberg
May 18, 2010·Current Opinion in Neurobiology·Loredana Zocchi, Paolo Sassone-Corsi
Jun 11, 2010·Nature·Catherine Dulac
Oct 12, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Angela Fontán-LozanoAngel Manuel Carrión
Nov 25, 2011·Methods in Molecular Biology·Badi Sri SailajaEran Meshorer
Dec 14, 2012·Proceedings of the National Academy of Sciences of the United States of America·Badi Sri SailajaEran Meshorer
Apr 10, 2013·Nature Reviews. Genetics·Jehnna L RonanGerald R Crabtree

❮ Previous
Next ❯

Citations

Jul 19, 2018·Oncotarget·Leonid Visochek, Malka Cohen-Armon
Sep 23, 2020·International Journal of Molecular Sciences·Alberto J LópezAndré O White
Jan 15, 2021·Neurochemistry International·Hitomi MatsunoKazuhiro Sohya
Dec 24, 2019·Journal of Molecular Biology·Haser H SutcuAlexander A Ishchenko
Nov 13, 2020·Scientific Reports·Sundararaghavan PattabiramanDaniel Kaganovich

❮ Previous
Next ❯

Methods Mentioned

BETA
immunoprecipitation
PCR
ChIP
salt extraction
transfections
transfection

Software Mentioned

ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Angela Fontán-LozanoAngel Manuel Carrión
Methods in Molecular Biology
J DélèzeJ C Hervé
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme
Y Kawasak
© 2022 Meta ULC. All rights reserved