Part I: characterization of the extracellular proteome of the extreme thermophile Caldicellulosiruptor saccharolyticus by GeLC-MS2.

Analytical and Bioanalytical Chemistry
Genna AndrewsDavid Muddiman

Abstract

The proteome of extremely thermophilic microorganisms affords a glimpse into the dynamics of microbial ecology of high temperature environments. The secretome, or extracellular proteome of these microorganisms, no doubt harbors technologically important enzymes and other thermostable biomolecules that, to date, have been characterized only to a limited extent. In the first of a two-part study on selected thermophiles, defining the secretome requires a sample preparation method that has no negative impact on all downstream experiments. Following efficient secretome purification, GeLC-MS(2) analysis and prediction servers suggested probable protein secretion to complement experimental data. In an effort to define the extracellular proteome of the extreme thermophilic bacterium Caldicellulosiruptor saccharolyticus, several techniques were considered regarding sample processing to achieve the most in-depth analysis of secreted proteins. Order of operation experiments, all including the C(18) bead technique, demonstrated that two levels of sample purification were necessary to effectively desalt the sample and provide sufficient protein identifications. Five sample preparation combinations yielded 71 proteins and the majority descri...Continue Reading

References

Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S SatoJ I Harris
Mar 1, 1990·Trends in Biochemical Sciences·A MueschT A Rapoport
Jul 1, 1980·Analytical Biochemistry·R Marusyk, A Sergeant
Apr 10, 1995·Analytical Biochemistry·D M SauvéM Roberge
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·G HarthM A Horwitz
Aug 1, 1997·Genome Research·M A BasraiJ D Boeke
Sep 15, 1998·Analytical Biochemistry·R L Winston, M C Fitzgerald
Sep 7, 2000·Microbiology and Molecular Biology Reviews : MMBR·H TjalsmaJ M van Dijl
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Lee R LyndIsak S Pretorius
Feb 3, 2004·Journal of Chromatography. a·Lei JiangMichael Fountoulakis
Apr 28, 2004·Journal of Molecular Biology·Lukas KällErik L L Sonnhammer
Jun 30, 2004·Journal of Molecular Biology·Jannick Dyrløv BendtsenSøren Brunak
Dec 16, 2004·Molecular & Cellular Proteomics : MCP·Karim RezaulDavid Han
Feb 11, 2005·Molecular & Cellular Proteomics : MCP·D Brent WeatherlyRon Orlando
Jun 28, 2005·Journal of the American Society for Mass Spectrometry·Ji GaoGregory J Opiteck
Jun 28, 2005·Molecular & Cellular Proteomics : MCP·William M OldNatalie G Ahn
Jul 5, 2005·BMC Bioinformatics·Jannick Dyrløv BendtsenSøren Brunak
Oct 11, 2005·BMC Microbiology·Jannick D BendtsenSøren Brunak
Sep 2, 2006·Journal of Proteome Research·Boris ZybailovMichael P Washburn
Apr 5, 2007·Nature Protocols·Andrej ShevchenkoMatthias Mann
Apr 12, 2007·Expert Review of Proteomics·Yetrib Hathout
Apr 28, 2007·Proteomics·Mireille ChevalletThierry Rabilloud
May 8, 2007·Nucleic Acids Research·Lukas KällErik L L Sonnhammer
Aug 2, 2007·Analytical and Bioanalytical Chemistry·Marcus BantscheffBernhard Kuster
Mar 29, 2008·BMC Bioinformatics·Miaomiao ZhouRoland J Siezen
Apr 2, 2008·Annals of the New York Academy of Sciences·Amy L VanfossenRobert M Kelly
Jun 6, 2008·Current Opinion in Biotechnology·Sara E Blumer-SchuetteRobert M Kelly
Sep 9, 2008·Applied and Environmental Microbiology·Harmen J G van de WerkenServé W M Kengen
Mar 10, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Genna L AndrewsDavid C Muddiman

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Citations

Apr 27, 2011·Environmental Microbiology·Dominic BurgRicardo Cavicchioli
Oct 15, 2013·FEMS Microbiology Reviews·Sara E Blumer-SchuetteRobert M Kelly
Oct 22, 2021·Molecular Biology Reports·Chandraprakash YaminiNarasimhan Manojkumar

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