Parthenolide facilitates apoptosis and reverses drug-resistance of human gastric carcinoma cells by inhibiting the STAT3 signaling pathway

Oncology Letters
Hua LiYuping Sun

Abstract

In the present study, SGC-7901/DDP cells were treated with different concentrations of parthenolide (PN) (2.5-15 µmol/l), cisplatin (DDP) (1.25-15 µg/ml) and PN+DDP. The proliferation inhibition rates were measured using an MTT assay, and the synergies of PN and DDP were analyzed. The effect of PN and DDP on SGC-7901/DDP cell proliferation demonstrated a time- and concentration-dependent association, and a synergy between PN and DDP was identified. DAPI staining and flow cytometry results indicated that 15 µmol/l PN significantly induced SGC-7901/DDP apoptosis and G1phase arrest compared with the untreated control group. Western blotting analysis results indicated that among the apoptosis-associated proteins, there were dose-dependent increases in the protein expression of apoptosis regulator BAX, cellular tumor antigen p53, cleaved caspase-3 and cleaved capase-9, and decreases in apoptosis regulator Bcl-2 and Bcl-xL protein expression levels. Among the cell cycle-associated proteins, cyclin D1 expression was significantly decreased, cyclin-dependent kinase inhibitor 1 expression was significantly increased, and signal transducer and activator of transcription 3 (STAT3) activation was inhibited. Scratch and Transwell assay resu...Continue Reading

References

Aug 4, 2005·Acta Biochimica Et Biophysica Sinica·Xiao-Yong LeiDuan-Fang Liao
Oct 20, 2006·Apoptosis : an International Journal on Programmed Cell Death·Wei WangYasuhisa Shinomura
Nov 23, 2006·Trends in Molecular Medicine·Benjamin BarréOlivier Coqueret
Jan 4, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yuki KishidaAkira Myoui
Jan 1, 2008·Journal of Oncology·Valentina CalderaDavide Schiffer
Feb 13, 2010·The Journal of Obstetrics and Gynaecology Research·Hao Min, Zhao Wei-hong
Jul 2, 2010·British Journal of Pharmacology·M CzyzM Wozniak
Aug 12, 2010·Journal of Experimental & Clinical Cancer Research : CR·Jun-Wei LiuDong-Sheng Huang
Mar 24, 2011·The Cancer Journal·Valentina FodaleEmanuel Petricoin
Aug 11, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Guang Cheng, Liping Xie
Apr 26, 2012·Molecular Cancer Therapeutics·Shinji KohsakaShinya Tanaka
May 24, 2012·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Bryan K HolcombC Max Schmidt
Oct 6, 2012·Cancer Prevention Research·Akram GhantousNadine Darwiche
Oct 8, 2014·Pharmaceutical Biology·Anees A Al-FatlawiAyaz Ahmad

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Citations

Jun 18, 2020·Biology·Milad AshrafizadehReza Mohammadinejad
Aug 3, 2019·International Journal of Molecular Sciences·Sabino RussiVitalba Ruggieri
Nov 30, 2019·Oxidative Medicine and Cellular Longevity·Elena ButturiniSofia Mariotto
Aug 23, 2020·Pharmaceuticals·Malgorzata Sztiller-Sikorska, Malgorzata Czyz
Dec 9, 2020·Journal of Medicinal Chemistry·Yulin Ren, A Douglas Kinghorn

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

SPSS
ImageJ

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