PMID: 20650058Feb 1, 1995Paper

Partial attenuation of hydroxyurea-induced embryotoxicity by deoxyribonucleotides in mouse and rat embryos treated in vitro

Toxicology in Vitro : an International Journal Published in Association with BIBRA
D K HansenS J James

Abstract

Hydroxyurea (HU) is a well known developmental toxicant in all animal species tested. It inhibits DNA synthesis, and addition of deoxycytidine monophosphate (dCMP) has been shown previously to attenuate the developmental toxicant effects of HU in vivo. The purpose of the present investigation was to determine whether addition of deoxyadenosine monophosphate (dAMP) or dCMP would attenuate HU-induced embryotoxicity using a rodent whole embryo culture system. Rat embryos were removed on the morning of day 10 of gestation, and mouse embryos were removed on day 8 of gestation (day 0 = the day a vaginal plug was found). Embryos were treated with various concentrations of HU (up to 500 mug/ml) for 1 hr at 37 degrees C before being washed and cultured for 43 hr in rat serum containing dAMP or dCMP. At the end of the culture period, six endpoints were evaluated for each viable embryo: morphological score; number of somite pairs; crown-rump and head lengths, DNA and protein contents. HU (300 mug/ml) significantly decreased values for all endpoints in embryos from both mice and rats; however, mouse embryos appeared to be more sensitive to the effects of the drug. dAMP and dCMP alone produced some embryotoxicity at high concentrations. The...Continue Reading

References

Feb 1, 1978·Biological Reviews of the Cambridge Philosophical Society·D A New
Apr 1, 1977·Teratology·J M DeSesso, R L Jordan
Jan 1, 1990·Reproductive Toxicology·J M DeSesso, G C Goeringer
Jan 1, 1988·Annual Review of Biochemistry·P Reichard
Jan 1, 1986·Advances in Experimental Medicine and Biology·P B RoweA Kalaizis
Dec 1, 1987·Teratology·T F GraftonD M Sheehan
Mar 1, 1980·Analytical Biochemistry·C Labarca, K Paigen
Mar 1, 1983·Molecular and Cellular Biology·E Wawra, E Wintersberger
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P B Rowe, S E McEwen
Aug 1, 1982·Journal of Toxicology and Environmental Health·L R DePass, E V Weaver
May 5, 1993·Journal of the National Cancer Institute·A E EskenaziC N Frantz
Nov 27, 1964·Science·C W YOUNG, S HODAS

❮ Previous
Next ❯

Citations

Aug 23, 2007·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Erica L LiebeltEdward J Stanek
Mar 1, 2014·Toxicological Research·Eui-Ju Hong, Eui-Bae Jeung
Dec 30, 2014·Environmental Toxicology and Pharmacology·Eui-Ju HongEui-Bae Jeung
Jun 24, 1999·Teratogenesis, Carcinogenesis, and Mutagenesis·T Platzek, R Schwabe
Oct 18, 2008·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Jianqun HanRuijuan Xiu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.