PMID: 6025317Jun 1, 1967Paper

Partial biochemical characterization of the activated esterase required in the complement-dependent chemotaxis of rabbit polymorphonuclear leukocytes

The Journal of Experimental Medicine
E L Becker, P A Ward

Abstract

It was shown in the preceding paper that incubation of the rabbit polymorphonuclearleukocyteswith phosphonate esters leads to an irreversible inhibition of the ability of the leukocyte to respond to the chemotactic factor. This "cell-dependent inhibition" was attributed to the inactivation by the phosphonates of an esterase existing in or on the leukocyte in an already activated state. As shown in this paper, incubating the leukocyte with phosphonate in the presence of certain esters prevents this cell-dependent inhibition. The protection is specific; the ester must be an acetate. Ethyl formate, ethyl propionate, ethyl butyrate, glucose 6-phosphate, fructose 1,6-diphosphate, ATP, tosyl arginine methyl ester, or acetyl tyrosine ethyl ester do not protect. The protection is independent of the phosphonate used to inhibit, and the degree of protection depends on the relative concentrations of acetate and phosphonate. Those acetates which protect are also the esters which inhibit chemotaxis when added to the leukocyte in the upper part of the chemotaxis chamber. It is concluded that the activated esterase is an enzyme capable of specifically splitting, or binding acetates, or doing both. Presumably the esterase is some type of acety...Continue Reading

References

Jun 1, 1967·The Journal of Experimental Medicine·P A Ward, E L Becker
Feb 1, 1964·Canadian Journal of Biochemistry·D J ECOBICHON, W KALOW

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Citations

Jun 1, 1983·Inflammation·M S Klempner, R E Rocklin
Jun 1, 1972·Agents and Actions·H U Keller
Jul 15, 1968·Experientia·H U Keller, E Sorkin
Oct 1, 1979·Food and Cosmetics Toxicology·D L Opdyke
Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S AswanikumarS M Wahl
Feb 1, 1973·The Journal of Cell Biology·J W DePierre, M L Karnovsky
Jun 1, 1967·The Journal of Experimental Medicine·P A Ward, E L Becker
Apr 1, 1968·The Journal of Experimental Medicine·P A Ward, E L Becker
Nov 1, 1968·The Journal of Experimental Medicine·P A Ward
Sep 1, 1969·The Journal of Experimental Medicine·J H Hill, P A Ward
Oct 1, 1969·The Journal of Experimental Medicine·D S PearlmanE L Becker
Nov 1, 1978·British Journal of Industrial Medicine·D Mangan, I S Snyder
Jan 1, 1970·Progress in Histochemistry and Cytochemistry·J Raekallio
Aug 16, 2008·Annual Review of Pathology·Peter A Ward
Oct 1, 1979·Clinical Immunology and Immunopathology·S K Ackerman, S D Douglas
Sep 1, 1970·Arthritis and Rheumatism
Oct 6, 1995·The Journal of Biological Chemistry·D A LomasR A Stockley
Aug 15, 1975·Klinische Wochenschrift·D NiethammerO Haferkamp
Jan 1, 1976·Immunological Communications·R N BoswellE J Goetzl
Mar 1, 1968·Biochemical Pharmacology·P A Ward
May 30, 1980·Biochemical and Biophysical Research Communications·J NiedelP Cuatrecasas
Mar 15, 1974·Science·J W DePierre, M L Karnovsky

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