Partial characterisation of citrus leaf blotch virus, a new virus from Nagami kumquat

Archives of Virology
Luis GalipiensoJosé Guerri

Abstract

Citrus leaf blotch virus (CLBV) was purified from leaves of Nagami kumquat SRA-153 that showed bud union crease when propagated on Troyer citrange. Virions were filamentous particles (960 x 14 nm) containing a 42 kDa protein and a single-stranded RNA (ssRNA) of about 9,000 nt (Mr 3 x 10(6)). Infected tissue contained three species of double-stranded RNA (dsRNA) of Mr 6, 4.5 and 3.4 x 10(6). The nucleotide sequence of several complementary DNA (cDNA) clones showed significant similarities with replication-related proteins from plant filamentous viruses in several genera. A digoxigenin-labelled probe from one of these cDNA clones hybridised in Northern blots with ssRNA from virions and with the three dsRNA species, suggesting that the ssRNA is the genomic RNA of the virus, the largest dsRNA is its replicative form, and the two smaller dsRNAs probably replicative forms of 5' co-terminal subgenomic RNAs. CLBV was also detected in several citrus cultivars from Spain and Japan including Navelina sweet orange field trees propagated on Troyer citrange showing bud union crease; however, no virus could be detected in other citrus trees with similar symptoms. This indicates that CLBV is not restricted to kumquat SRA-153, but its involveme...Continue Reading

Citations

Dec 31, 2008·Archives of Virology·M RussoGiovanni P Martelli
May 18, 2012·Archives of Virology·Luisa RubinoGiovanni P Martelli
Mar 16, 2013·Archives of Virology·Ramesh R ChavanMichael N Pearson
Sep 29, 2011·Virus Genes·Águeda RenovellJosé Guerri
Mar 17, 2007·Annual Review of Phytopathology·Giovanni P MartelliValerian V Dolja
May 2, 2009·Journal of Virological Methods·Susana Ruiz-RuizJosé Guerri
Nov 21, 2008·Molecular Plant Pathology·María Carmen VivesJosé Guerri
Apr 9, 2013·Molecular Plant Pathology·Jesús AgüeroJosé Guerri

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