PMID: 6167986Jun 1, 1981Paper

Partial characterization of gamma (immune) interferon mRNA extracted from human lymphocytes

Proceedings of the National Academy of Sciences of the United States of America
T TaniguchiJ Vilcek

Abstract

gamma (immune) interferon (IFN-gamma) was induced in cultures of fresh human lymphocytes by combined treatment with a phorbol ester (12-O-tetradecanoylphorbol 13-acetate, TPA) and the T cell mitogen phytohemagglutinin (PHA). Compared to the IFN-gamma yields obtained with PHA induction alone, the inclusion of TPA caused a significant enhancement of IFN-gamma production. Poly(A)-containing mRNA was isolated from mononuclear cells induced with TPA and PHA. Injected into Xenopus laevis oocytes, this mRNA preparation gave rise to IFN activity with characteristic properties of human IFN-gamma. Sucrose density gradient centrifugation analysis showed that IFN-gamma mRNA sedimented at 15 S, suggesting that it contains a total of about 1400 nucleotides.

References

Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M N ThangL Montagnier
Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R L CavalieriS Pestka
Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·P B SehgalI Tamm
Sep 15, 1978·Cellular Immunology·S B MizelJ J Oppenheim
Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S PestkaJ Vilcek
May 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J de Maeyer-GuignardL Montagnier
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R H PangJ Vilcek
May 1, 1980·Molecular Immunology·M Wiranowska-StewartW E Stewart
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·Y K YipJ Vilcek

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Citations

Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T FujitaT Taniguchi
Oct 1, 1987·Journal of Interferon Research·T Taniguchi
Jul 27, 2007·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Jan Vilcek
Jul 15, 1985·European Journal of Biochemistry·H D CampbellI G Young
Sep 30, 1982·Biochimica Et Biophysica Acta·P B Sehgal
Apr 29, 1982·Biochemical and Biophysical Research Communications·A FuseA Billiau
Jan 15, 1982·Biochemical and Biophysical Research Communications·H WeeningA Billiau
Aug 1, 1991·Clinical and Experimental Immunology·N YoshimuraJ Imanishi
Oct 1, 1981·Infection and Immunity·Y K YipJ Vilcek

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