Partial characterization of lipid A of intraperiplasmically grown Bdellovibrio bacteriovorus.

Journal of Bacteriology
D R Nelson, S C Rittenberg

Abstract

The lipid A components of substrate cell origin incorporated by Bdellovibrio bacteriovorus during intraperiplasmic growth (D. R. Nelson and S. C. Rittenberg, J. Bacteriol. 147:860-868, 1981) were shown to be integrated into its lipopolysaccharide structure. Lipid A isolated from bdellovibrios grown on Escherichia coli was resolved into two fractions by thin-layer chromatography. Fraction 2 had the same Rf as the single lipid A fraction of axenicaly grown bdellovibrios, and both stained identically with aniline-diphenylamine reagent. Fraction 1 resembled, in Rf and staining reaction, the slower migrating of two lipid A fractions obtained from the E.coli used as the substrate cell. Both fractions 1 and 2 contained glucosamine, a substrate cell-derived compound. Greater than 65% of the fatty acids in fraction 1 were derived from the substrate cell, whereas more than 60% of the fatty acids of fraction 2 were synthesized by the bdellovibrio. Nevertheless, each fraction contained significant amounts of fatty acid of both origins. The substrate cell-derived fatty acids had the same distribution of N-acyl and O-acyl linkages as in E. coli lipid A. The data indicate that the two lipid A moieties in lipopolysaccharide of intraperiplasmic...Continue Reading

References

Mar 17, 1975·European Journal of Biochemistry·S G Wilkinson, L Galbrath
Jul 13, 1972·European Journal of Biochemistry·E T RietschelO Westphal
Jun 1, 1969·European Journal of Biochemistry·C GalanosO Westphal
Mar 30, 1956·Science·S Schwimmer, A Bevenue

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Citations

Nov 30, 2005·Molecular Microbiology·Ian E GentleTrevor Lithgow
Sep 1, 1985·Journal of Bacteriology·L S Thomashow, S C Rittenberg
Aug 1, 1982·Journal of Bacteriology·D A OdelsonR B Hespell

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