PMID: 8594414Jul 19, 1995Paper

Partial characterization of the RNA from LPS-stimulated macrophages that induces the release of chemotactic cytokines by resident macrophages

Molecular and Cellular Biochemistry
R A RibeiroF L De Lucca

Abstract

It is well established that exogenous RNA is incorporated into eukaryotic cells and is able to exert various biological responses. Little, however, is known about the effects of such RNA on macrophages. In this study, we demonstrate that RNA extracted from macrophages stimulated with Escherichia coli lipopolysaccharide (LPS), referred to as L-RNA, in contrast to RNA from non-stimulated macrophages (N-RNA), induces the release of a macrophage-derived neutrophil chemotactic factor (MNCF) and interleukin-8 (IL-8) from macrophage monolayers. The effect of L-RNA was dependent of the integrity of the polynucleotide chain and was not due to LPS contamination since its ability to induce MNCF and IL-8 release was strongly reduced by RNase but was not affected by DNase or polymyxin B. The poly A(+)L-RNA and poly A(-)L-RNA fractions were able to induce the release of MNCF and IL-8, indicating that the L-RNA could be acting at transcriptional and translational levels. The demonstration that actinomycin-D and cycloheximide inhibited the release of MNCF and IL-8 by L-RNA-stimulated macrophages confirms this assumption. Fractionation of the total L-RNA by centrifugation on a 5-20% sucrose gradient showed that the L-RNA which sediments in the ...Continue Reading

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Citations

Apr 12, 2003·European Journal of Pharmacology·Maria Angelica Ehara WatanabeFernando Luiz De Lucca
Nov 1, 2001·Clinical and Diagnostic Laboratory Immunology·G U MeduriD R Schaberg
Dec 16, 2006·Naunyn-Schmiedeberg's Archives of Pharmacology·Nylane M N AlencarBenildo S Cavada
Jun 13, 2009·Toxicon : Official Journal of the International Society on Toxinology·Jozi G FigueiredoNylane M N Alencar
Oct 12, 2007·Blood Cells, Molecules & Diseases·Nayara Delgado André, Fernando L De Lucca
Jul 20, 2005·Blood Cells, Molecules & Diseases·Marla Karine AmaranteMaria Angelica Ehara Watanabe

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