PMID: 2501263Mar 1, 1989Paper

Partial immunological characterization of heat-stable proteinases from Pseudomonas spp. of dairy origin

The Journal of Applied Bacteriology
J I AzconaB Sanz

Abstract

A homogeneous extracellular heat-stable proteinase from Pseudomonas fluorescens AH-70 was used to prepare antiserum in rabbits. The rabbit antiserum was used to study the antigenic relationship of the proteinases from 26 psychrotrophic Pseudomonas spp. isolated from raw milk. The inhibition of the proteinases by the antiserum, the gel precipitin reactions and the use of a double-antibody sandwich ELISA, revealed that proteinase AH-70 is immunologically related to proteinases from 8/26 other Pseudomonas strains. These results also indicate that the immunological techniques for the detection of proteolytic enzymes in raw milk require antibody preparations of broader specificity.

References

Jun 1, 1975·The Journal of Dairy Research·H B Castberg, P Solberg
Jun 16, 1967·Science·L A Heppel
May 27, 1969·Biochimica Et Biophysica Acta·C J Fielding

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Citations

Jan 1, 1991·Critical Reviews in Food Science and Nutrition·U SamarajeewaM R Marshall
Jul 25, 2007·Journal of Agricultural and Food Chemistry·Didier DupontJacques Degelaen

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