PMID: 108668Mar 1, 1979

Partial mapping of methylated sequences in Xenopus laevis ribosomal RNA by preparative hybridization to cloned fragments of ribosomal DNA

Nucleic Acids Research
B E Maden, R H Reeder

Abstract

Xenopus laevis rRNA was hybridised to either of two cloned fragments of ribosomal DNA. One fragment, designated X1r11, contains a short region of the 18 S rRNA gene and most of the 28 S rRNA gene. The other fragment, X1r14, contains a short region of the 28 S gene and most of the 18 S gene. After hybridization the non-complementary rRNA was removed by digestion with T1 RNase and the hybridized RNA was then eluted and examined by fingerprinting analysis. The 3' terminal sequence and the dimethyl-A-containing sequence of 18 S rRNA both hybridized to X1r11 rDNA, in agreement with the known direction of transcription of rDNA. The distribution of other methylated oligonucleotides between the various fingerprints permitted assigment of nearly all of the methylated sequences in 18 S and 28 s rRNA to either the short 3' region or the long 5' region of the respective molecules.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R H ReederH L Wahn
May 2, 1977·European Journal of Biochemistry·R C BrandR J Planta
Oct 1, 1977·Nucleic Acids Research·P De JongeR J Planta
Apr 1, 1978·European Journal of Biochemistry·J K Vass, B E Maden
Apr 1, 1978·Nucleic Acids Research·P G BoseleyM L Birnstiel
Aug 25, 1976·Journal of Molecular Biology·P K WellauerR H Reeder
Sep 5, 1974·Journal of Molecular Biology·B E Maden, M Salim
Jul 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·P K WellauerI B Dawid
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J F MorrowR B Helling
Nov 15, 1973·European Journal of Biochemistry·J Klootwijk, R J Planta
Jul 1, 1965·Journal of Molecular Biology·D Gillespie, S Spiegelman

Citations

Jan 1, 1986·Bio Systems·S A Gerbi
Dec 16, 1998·Trends in Biochemical Sciences·B E Maden
Nov 11, 1980·Nucleic Acids Research·R J Baer, D T Dubin

Related Concepts

Recombination Joint
RNase Pch 1
Methylation
Genomic Hybridization
Oligoribonucleotides
Ribonucleotides
Ribosomes
15S RNA

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.