Abstract
The nucleotide sequence of a Salmonella typhimurium DNA segment of 549 base pairs which encompasses the operator-promoter of the pts operon, the entirety of the ptsH gene, encoding HPr of the phosphotransferase system (PTS), the first 29 nucleotides of the ptsI gene, encoding Enzyme I of the PTS, and the intercistronic region between the ptsH and ptsI genes was determined and compared with the corresponding sequence from Escherichia coli (De Reuse et al., 1985). The two sequences showed 91% overall identity, with some regions showing sequence conservation and others exhibiting relative divergence. Two open reading frames were identified in both species: one encoded HPr on the 'sense' strand (255 nucleotides; 12 nucleotide differences, no amino acid differences); the other, on the anti-sense strand, consisted of 291 nucleotides (13 nucleotide differences, 13 amino acid differences). While HPr bears a net negative charge, the putative protein encoded by the open reading frame on the anti-sense strand is strongly basic. Computer analyses of HPr proteins from five different bacterial genera revealed four regions which show strong sequence identity and therefore are presumed to be critical for maintenance of biological activity. Two...Continue Reading
References
May 2, 1977·European Journal of Biochemistry·K BeyreutherW Hengstenberg
May 2, 1977·European Journal of Biochemistry·M GassnerH Rüterjans
Jan 1, 1985·Gene·H De ReuseA Danchin
Sep 1, 1988·Journal of Bacteriology·H De Reuse, A Danchin
Jan 1, 1989·Molecular Microbiology·G Gonzy-TréboulM Steinmetz
Mar 1, 1988·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M H SaierC A Lee
Jan 1, 1988·Critical Reviews in Microbiology·J ReizerW Hengstenberg
Jan 1, 1987·Journal of Molecular Evolution·D F Feng, R F Doolittle
May 1, 1988·Molecular Microbiology·T DavisM H Saier
May 1, 1988·Journal of Bacteriology·M H Saier, P McCaldon
Nov 18, 1986·Biochemistry·R E Klevit, E B Waygood
Sep 25, 1981·Journal of Molecular Biology·T Ikemura
Oct 25, 1980·Journal of Molecular Biology·F SangerB A Roe
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J Deutscher, M H Saier
Citations
Aug 11, 1991·Journal of Molecular Evolution·A ReizerM H Saier
Jun 1, 1989·FEMS Microbiology Reviews·J Reizer
Sep 1, 1989·Biochimie·J ReizerM H Saier
Dec 22, 1993·Gene·G GagnonM Frenette
Jul 1, 1989·Research in Microbiology·M H Saier
Mar 11, 1990·Nucleic Acids Research
Apr 11, 1990·Nucleic Acids Research·F TitgemeyerJ W Lengeler
Feb 1, 1993·European Journal of Biochemistry·H Sakai, T Ohta
Feb 23, 2017·Scientific Reports·Mangyu ChoeYeong-Jae Seok
Mar 5, 1990·Journal of Molecular Biology·G KapadiaO Herzberg
Jun 20, 1990·Journal of Molecular Biology·L F WuM H Saier
Jan 1, 1989·Molecular Microbiology·G Gonzy-TréboulM Steinmetz
Jun 1, 1995·Molecular Microbiology·G GagnonM Frenette
Dec 3, 2008·Journal of Bacteriology·Jennifer K WagnerMarcia B Goldberg
Aug 1, 1993·FEMS Microbiology Letters·C VadeboncoeurS Gingras
Jun 1, 1995·Microbiological Reviews·K E SandersonK E Rudd
Jun 1, 1997·Applied and Environmental Microbiology·R StentzM Zagorec
Sep 1, 1991·Journal of Bacteriology·A PriesA Steinbüchel
Mar 1, 1994·Microbiological Reviews·M H Saier
Sep 1, 1993·Microbiological Reviews·P W PostmaG R Jacobson