May 1, 1976

Partial proteolysis of some cellulase components from Trichoderma viride and the substrate specificity of the modified products

Journal of Biochemistry
M NakayamaK Nisizawa

Abstract

An endo-cellulase component [EC 3.2.1.4] or random type, F II, was obtained from "Cellulase Onozuka," a commercial product from Trichoderma viride, and was subjected to partial proteolysiats with a protease preparation of the same fungal origin. The resulting modified cellulase was fractioned by two steps of column chromatography, and the resulting patterns, together with the substrate specificity expressed in terms of the randomness of CMC hydrolysis and the immunological properties against anti-F II-rabbit se-um, were examined. The chromatographic patterns were very similar to those of cellulase subfractions without proteolytic treatment. Moreover, the immunological response of the modified cellulases from F II was mostly positive and their randomness of CMC hydrolysis was generally lower, compared with subfractions of F II which were not subjected to proteolysis. The subfractions of Peak III, which were obtained from F II by proteolysis, showed mostly negative immunological response and higher randomness of CMC hydrolysis compared with subfractions of Peak III which were not subjected to proteolysis. Thus, some limited proteolysis of cellulase components may, at least in part, be responsible for its multiplicity in vivo.

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Mentioned in this Paper

In Vivo
Patterns
Candidiasis, Chronic Mucocutaneous
Other and Unspecified Abnormality of Organs and Soft Tissues of Pelvis, Unspecified as to Episode of Care or Not Applicable
Peptide Hydrolases
MANEA
Fungi
Capillary Malformation (Disorder)
Proteolysis
Exoglycosidases

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