PMID: 6714234Apr 16, 1984

Partial purification and characterization of a 60 000-dalton phosphoprotein from pig heart tissue

European Journal of Biochemistry
T David-Pfeuty, A Hovanessian

Abstract

A 60 000-dalton phosphoprotein (pp60) was purified up to 10(4)-fold by a combination of low-ionic-strength extraction, ammonium sulfate fractionation, on-exchange and affinity chromatography, all in detergent-free buffer. Fractionation on omega-aminohexylagarose column shows that pp60 actually consists of two different polypeptides of similar molecular mass (pp60 omega 1 and pp60 omega 2). Partial hydrolysis with proteases of the proteins 32P-labeled in vitro indicates that pp60 omega 1 and pp60 omega 2 are similar but not identical. On the other hand, individual phosphoamino acid analysis reveals that pp60 omega 1 is phosphorylated primarily at serine residues while pp60 omega 1 is phosphorylated almost equally at serine and threonine residues. Partial hydrolysis with proteases has been also used to explore a possible relationship between the pp60's and the transforming protein of Rous sarcoma virus (pp60v-src). Our data suggest that pp60v-src also consists of two different polypeptides chemically homologous to the presently purified pp60's.

References

Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A F PurchioR L Erikson
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A F PurchioR L Erikson
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H OppermannJ M Bishop
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R L EriksonA F Purchio
Feb 18, 1972·Science·S J Singer, G L Nicolson
Oct 22, 1981·Nature·D J Shealy, R L Erikson
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T Hunter, B M Sefton
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Citations

Mar 1, 1989·Biochimie·F Guesdon, T David-Pfeuty
Jun 15, 1987·Biochemical and Biophysical Research Communications·T David-Pfeuty, F Guesdon
Aug 28, 1990·European Journal of Biochemistry·T David-Pfeuty, Y Nouvian-Dooghe

Related Concepts

Chromatography, DEAE-Cellulose
Hydrolysis
Immunochemistry
Myocardium
Phosphoproteins
Phosphorylation

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