Partial Purification and Characterization of a Bacteriocin DT24 Produced by Probiotic Vaginal Lactobacillus brevis DT24 and Determination of its Anti-Uropathogenic Escherichia coli Potential

Probiotics and Antimicrobial Proteins
Disha TrivediSriram Seshadri

Abstract

The emergence of antibiotic resistance has increased the interest for finding new antimicrobials in the past decade. Probiotic Lactic acid bacteria producing antimicrobial proteins like bacteriocin can be excellent agents for development as novel therapeutic agents and complement to conventional antibiotic therapy. Uropathogenic Escherichia coli, most causative agent of Urinary tract infection, has developed resistance to various antibiotics. In the present investigation, antibacterial substance like bacteriocin (Bacteriocin DT24) produced by probiotic Lactobacillus brevis DT24 from vaginal sample of healthy Indian woman was partially purified and characterized. It was efficiently working against various pathogens, that is, Uropathogenic E. coli, Enterococcus faecium, Enterococcus faecalis and Staphylococcus aureus. The antimicrobial peptide was relatively heat resistant and also active over a broad range of pH 2-10. It has been partially purified by ammonium sulfate precipitation and gel filtration chromatography and checked on reverse-phase high-performance liquid chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of bacteriocin DT24 was approximately 7-kDa protein. The peptide is inactivated by proteol...Continue Reading

References

Dec 1, 1989·Annals of Internal Medicine·J R Johnson, W E Stamm
Sep 1, 1993·FEMS Microbiology Reviews·T R Klaenhammer
Oct 1, 1996·Journal of Clinical Microbiology·J D Sobel, W Chaim
Apr 1, 1999·International Journal of Food Microbiology·M G GänzleW P Hammes
Jul 8, 1999·International Journal of Antimicrobial Agents·K GuptaW E Stamm
Aug 5, 2000·Oral Diseases·M E CollocaM E Nader-Macías
Dec 21, 2000·Letters in Applied Microbiology·B Del ReD Palenzona
Feb 7, 2001·The American Journal of Clinical Nutrition·C DunneJ K Collins
Feb 7, 2001·The American Journal of Clinical Nutrition·G Reid
Mar 15, 2001·International Journal of Food Microbiology·M C AudisioM C Apella
May 23, 2001·Infectious Diseases in Obstetrics and Gynecology·A A AroutchevaS Faro
Jul 13, 2002·The American Journal of Medicine·Allan Ronald
Nov 9, 2002·Biochimie·Sylvie GarneauJohn C Vederas
Aug 21, 2003·International Journal of Food Microbiology·Inga Marie AasenKristine Naterstad
Jan 11, 2005·Current Protein & Peptide Science·Paul D CotterR Paul Ross
Jul 20, 2005·Journal of Basic Microbiology·Svetoslav D Todorov, Leon M T Dicks
Oct 6, 2005·Nature Reviews. Microbiology·Paul D CotterR Paul Ross
Apr 25, 2006·Journal of Food Protection·Virginia A StergiouMartin R Adams
Sep 13, 2006·FEMS Immunology and Medical Microbiology·Jasadee KaewsrichanJaturavit Kongprasertkit
Dec 1, 2006·Applied Microbiology and Biotechnology·Pyoung Il KimYong-Ha Park
Jun 30, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Christopher A CzajaWalter E Stamm
Jun 30, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Betsy FoxmanPatricia Brown
Aug 28, 2007·Journal of Microbiological Methods·M Carmen ColladoSeppo Salminen
Mar 12, 2009·Applied Biochemistry and Biotechnology·Manoj KumarSheela Srivastava
Mar 18, 2009·Microbiology and Immunology·Havva EkmekciSahlan Ozturk
Aug 7, 2010·Food Microbiology·Svetoslav Dimitrov TodorovDjamel Drider
Feb 15, 2011·International Journal of Food Microbiology·Carmen Nueno-Palop, Arjan Narbad
May 1, 1993·Journal of Food Protection·Maria Elena Nader DE MacíasGuillermo Oliver

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Citations

Jan 31, 2015·Probiotics and Antimicrobial Proteins·Tapasa Kumar SahooSriram Seshadri
Jan 8, 2017·Probiotics and Antimicrobial Proteins·Tapasa Kumar SahooSriram Seshadri
Jun 22, 2021·Critical Reviews in Food Science and Nutrition·Amin AbbasiAziz Homayouni-Rad
Oct 4, 2020·Microbial Pathogenesis·Stylianos VagiosCaroline Mitchell

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