Partial purification and characterization of a protein kinase that is activated by nuclear localization signal peptides

FEBS Letters
T KuriharaY Yoneda

Abstract

A nuclear localization signal (NLS) is required for the transport of karyophilic proteins from the cytoplasm to the nucleus. In this study, NLS was examined in terms of its effect on diverse cellular functions such as protein phosphorylation reactions. When synthetic peptides containing the NLS of SV40 T-antigen were injected into the cytoplasm of Xenopus oocytes, and the oocytes incubated with [32P]phosphorous-containing medium, a 32 kDa protein was found to be preferentially phosphorylated in an NLS-dependent manner. The incubation of fractionated cytosolic extracts prepared from mouse Ehrlich ascites tumor cells with [gamma-32P]ATP in the presence of the NLS peptides, results in the stimulation of the phosphorylation of several proteins. Similar in vitro stimulation was observed by other functional NLS peptides such as those of polyoma virus T-antigen and nucleoplasmin. Little or no stimulation, however, was detected for peptides of mutant type and reverse type NLS of SV40 T-antigen, and the C-terminal portion of lamin B. Using an in vitro assay, the phosphorylation activity was fractionated chromatographically and a fraction was obtained which contained a high level of activity. The fraction was found to contain three major...Continue Reading

References

Aug 1, 1992·Molecular and Cellular Biology·M DassoJ W Newport
Dec 1, 1991·Trends in Biochemical Sciences·C Dingwall, R A Laskey
Feb 8, 1991·Cell·P A Silver
Mar 7, 1991·Biochimica Et Biophysica Acta·J Garcia-BustosM N Hall
May 1, 1987·The Journal of Cell Biology·C M SnowL Gerace
Aug 14, 1986·Nature·D S GoldfarbR D Kornberg
Jul 1, 1995·The Journal of Cell Biology·N C ChiS A Adam
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·A RaduM S Moore
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·J MoroianuA Radu
Dec 16, 1994·Cell·M A Powers, D J Forbes
Jul 1, 1991·Trends in Cell Biology·D Goldfarb, N Michaud
May 1, 1992·Trends in Cell Biology·L Yamasaki, R E Lanford

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