Partial purification and characterization of a gibberellin-binding protein from seedlings of Azukia angularis

Biochemical and Biophysical Research Communications
M NakajimaN Murofushi

Abstract

Little is known about mechanisms for the perception of gibberellin (GA), a phytohormone, by plants. A GA-binding protein (GBP) with receptor-like characteristics was partially purified from azuki bean (Azukia angularis) seedlings. Gibberellins that caused much elongation of azuki epicotyl sections showed strong binding affinity for the GBP, and their biosynthetic precursors and inactive metabolites had weak affinity for it. The apparent Kd value for GA4 was calculated to be 7 x 10(-10) M from Scatchard plots. The molecular weight of the GBP in the native state was estimated to be 25,000 by high-performance liquid chromatography with a gel-permeation column. The GBP may be one of the receptor candidates for GAs.

Citations

May 3, 2007·Annual Review of Plant Biology·Miyako Ueguchi-TanakaMakoto Matsuoka
Oct 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·M Ueguchi-TanakaM Matsuoka
Mar 2, 2016·Bioscience, Biotechnology, and Biochemistry·Isomaro YamaguchiSeung-Hyun Park
Sep 30, 2005·Nature·Miyako Ueguchi-TanakaMakoto Matsuoka
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Jul 19, 2017·Bioorganic & Medicinal Chemistry Letters·Kai JiangTadao Asami
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