Partial purification and characterization of bacteriocin produced by Enterococcus faecalis DU10 and its probiotic attributes

Preparative Biochemistry & Biotechnology
Venkatesh PerumalArul Venkatesan

Abstract

A novel bacteriocin produced by avian duck isolated lactic acid bacterium Enterococcus faecalis DU10 was isolated. This bacteriocin showed a broad spectrum of antibacterial activity against important food-borne pathogens and was purified by size exclusion chromatography followed by reverse-phase high-performance liquid chromatography in a C-18 column. Tricine-SDS PAGE revealed the presence of a band with an estimated molecular mass of 6.3 kDa. The zymogram clearly linked the antimicrobial activity with this band. This result was further confirmed by mass-assisted laser desorption ionization time-of-flight mass spectrometry, since a sharp peak corresponding to 6.313 kDa was detected and the functional groups were revealed by Fourier transform infrared spectroscopy. Bacteriocin DU10 activity was found sensitive to proteinase-K and pepsin and partially affected by trypsin and α-chymotrypsin. The activity of bacteriocin DU10 was partially resistant to heat treatments ranging from 30 to 90°C for 30 min. It also withstood a treatment at 121°C for 10 min. Cytotoxicity of bacteriocin DU10 by methyl-thiazolyl-diphenyl-tetrazolium bromide assay showed that the viability of HT-29 and HeLa cells decreased 60 ± 0.7% and 43 ± 4.8%, respectiv...Continue Reading

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Citations

Sep 12, 2017·Critical Reviews in Microbiology·Gayatri SureshStephane Godbout
Jul 7, 2020·Microbial Pathogenesis·Likaa Hamied MahdiIbtesam Ghadban Auda
Dec 2, 2021·Journal of Clinical Laboratory Analysis·Atieh DarbandiMaryam Kakanj

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Datasets Mentioned

BETA
KJ622043

Methods Mentioned

BETA
size dialysis
infrared spectroscopy
ELISA
size exclusion chromatography

Software Mentioned

OriginPro

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