PMID: 6165014Mar 1, 1981Paper

Partial purification and characterization of human gamma (immune) interferon

Proceedings of the National Academy of Sciences of the United States of America
Y K YipJ Vilcek

Abstract

Human gamma (immune) interferon (IFN-gamma) was produced in lymphocyte cultures stimulated with a phorbol ester (12-O-tetradecanoylphorbol 13-acetate) and purified phytohemagglutinin. Physicochemical analysis showed that human IFN-gamma is a glycoprotein with an isoelectric point around 8.6 and an apparent molecular weight of 58,000 +/- 3000. A purification process for IFN-gamma was developed consisting of sequential chromatographic separations on controlled-pore glass, concanavalin A-Sepharose, and Bio-Gel P-200. This purification process resulted in an increase in specific activity from about 10(4) (crude culture fluid) to an estimated 10(7) units per mg of protein with a cumulative recovery of about 40% of the IFN activity.

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Citations

Jun 26, 1992·Journal of Chromatography·Z ZhangJ C Janson
Jan 1, 1982·Journal of Immunological Methods·H KirchnerW Digel
Apr 21, 1989·Journal of Immunological Methods·T VolgmannH Mohr
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Mar 14, 2000·Veterinary Immunology and Immunopathology·C H YunK D Choi
Mar 18, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·S Kudo, K Kawano
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T TaniguchiJ Vilcek
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·Y K YipJ Vilcek
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S J ScahillW Fiers
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T FujitaT Taniguchi
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R FukunagaS Nagata
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·J A LangerE Kempner
Jan 1, 1981·Preparative Biochemistry·K C Chadha, E Sulkowski
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Dec 15, 2010·Hepatitis Research and Treatment·Robert M Friedman, Sara Contente

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