PMID: 6171741Aug 1, 1981Paper

Partial purification and characterization of neutral proteases in lymph nodes of rats with experimental allergic encephalomyelitis

Neurochemical Research
M E SMithR H Rolph

Abstract

Two kinds of neutral protease activities in lymph nodes from Lewis rats with acute experimental allergic encephalomyelits (EAE) have been separated and partially purified and characterized. A soluble enzyme preparation enriched by gel filtration and ion-exchange chromatography hydrolyzes myelin basic protein, polylysine, and other basic proteins with an optimum pH at 6.0-6.5. It is inhibited by p-chloromercuribenzoate, and thus appears to be a mixture of thiol proteases. Another fraction containing proteolytic enzyme activity is strongly bound to the insoluble lymph node residue, and it also hydrolyzes myelin basic protein and histone, but not polylysine. It has a pH optimum above 7.5, is inhibited by phenylmethylsulfonyl fluoride, thus resembling elastase, but does not hydrolyze elastin-Congo red. The insoluble enzyme preparation hydrolyzes basic protein to 4-5 peptides in a pattern on polyacrylamide gels resembling that of the hydrolysis of basis protein by whole lymphocytes; the soluble enzyme mixture produces small fragments not retained on gels. Lymphocytes are a major component of the cells infiltrating the nervous system in experimental allergic encephalomyelitis, and neutral proteases contained in these cells may contri...Continue Reading

References

Jan 1, 1978·Advances in Experimental Medicine and Biology·M E SMith
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·W CammerS Gordon
Jul 1, 1975·Cellular Immunology·A I GrayzelG S Lazarus
Oct 22, 1968·Biochimica Et Biophysica Acta·M E Smith
Nov 1, 1969·The Histochemical Journal·J F Hallpike, C W Adams
Feb 21, 1967·Biochimica Et Biophysica Acta·K R Woods, K T Wang
Nov 1, 1976·Journal of Neurochemistry·M E Smith
Jan 1, 1972·Methods in Enzymology·W R Gray

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Citations

May 1, 1984·Neurochemical Research·K YanagisawaN Katsunuma
Jun 1, 1985·Neurochemical Research·M BenuckA Lajtha
Mar 1, 1986·Neurochemical Research·J Trotter, M E Smith
Dec 1, 1984·Journal of Neurochemistry·M E SmithJ Trotter
May 1, 1988·Journal of Neurochemistry·C M WatsonA N Davison

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