PMID: 6407525Feb 7, 1983Paper

Partial purification and characterization of arachidonate 12-lipoxygenase from rat lung

Biochimica Et Biophysica Acta
C YokoyamaC R Pace-Asciak

Abstract

Incubation of rat lung cytosol with arachidonic acid produced 12-hydroxy-5,8,10,14-eicosatetraenoic acid as a major product, which was identified by gas chromatography-mass spectrometry. By ammonium sulfate fractionation and DEAE-cellulose chromatography the arachidonate 12-lipoxygenase was purified about 30-fold from the rat lung cytosol. The partially purified enzyme was mostly free of the glutathione peroxidase activity and transformed arachidonic acid to its 12-hydroperoxide. 5,8,11,14,17-Eicosapentaenoic acid was also an active substrate, and the oxygenation at C-12 was confirmed by mass spectrometry. A significant amount of 12-lipoxygenase activity was also found in the microsomes and other particulate fractions.

References

Nov 1, 1979·Prostaglandins and Medicine·B R CulpW E Lands
Aug 1, 1979·The Journal of Experimental Medicine·E J Goetzl, F F Sun
Feb 20, 1975·Biochimica Et Biophysica Acta·D H Nugteren
May 23, 1976·Biochemical and Biophysical Research Communications·P P HoH R Sullivan
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M Hamberg, B Samuelsson
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M I SiegelP Cuatrecasas
Aug 24, 1981·Biochimica Et Biophysica Acta·C R Pace-AsciakS Yamamoto
Feb 11, 1982·Biochemical and Biophysical Research Communications·S PiloteP Borgeat

❮ Previous
Next ❯

Citations

Feb 1, 1992·The International Journal of Biochemistry·A P KulkarniI S Richards
Feb 1, 1984·Prostaglandins·T TeranoS Moncada
Jan 1, 1984·Prostaglandins, Leukotrienes, and Medicine·W E Lands
Oct 1, 1995·Journal of Lipid Mediators and Cell Signalling·T Yoshimoto, S Yamamoto
May 21, 1999·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·J W HollandA F Rowley
Feb 1, 1985·The Journal of Clinical Investigation·M A Warso, W E Lands
May 1, 1992·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·J Z Byczkowski, A P Kulkarni
Jan 1, 1990·Archives of Oral Biology·K TamaiA Tsujimoto
Jan 1, 1994·Journal of Ocular Pharmacology·T AsakuraH Shichi
Jun 24, 1999·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·P Roy, A P Kulkarni
Oct 1, 1989·Alimentary Pharmacology & Therapeutics·T B McCallC A O'Moráin
Nov 15, 1985·Biochemical Pharmacology·M StüningW König
Aug 6, 1986·Biochimica Et Biophysica Acta·S FlatmanT F Slater
Jan 1, 1988·Progress in Lipid Research·A A SpectorS A Moore
Aug 1, 1984·Prostaglandins·T YoshimotoF Dray
Apr 1, 1994·Prostaglandins, Leukotrienes, and Essential Fatty Acids·K A Akhilender NaiduA P Kulkarni
Nov 1, 1986·Pharmacological Research Communications·M RadomskiR J Gryglewski
Feb 28, 1985·Biochemical and Biophysical Research Communications·A BabaH Iwata

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.