Partial purification of a Bacillus licheniformis levansucrase producing levan with antitumor activity

International Journal of Biological Macromolecules
Imen DahechHafedh Mejdoub

Abstract

The extracellular fructosyltransferase (FTase) of a novel strain of Bacillus licheniformis capable of producing fructooligosaccharides (FOS) and a polysaccharide type levan was obtained and partially purified. The purification was achieved by ammonium sulfate precipitation, DEAE cellulose and gel filtration chromatographies. The enzyme was partially purified as determined by SDS-PAGE, and the specific activity reached was 67.5, representing a purification factor of 177 and yield of 40%. Levan was isolated from the cultures of B. licheniformis. The levan was composed mainly of fructose residues when analyzed by TLC after acid hydrolysis and NMR analysis. In a previous study, the levan produced exhibited a hypoglycemiant activity. The present paper deals with the study of the antitumor and anti-cytotoxic effect of levan produced by B. licheniformis strain. In the in vitro antitumor activity test of levan against some tumor cell lines, relatively the significantly high activity was observed against the HepG(2).

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Sep 20, 2011·International Journal of Biological Macromolecules·Imen DahechHafedh Mejdoub
Jan 12, 2012·International Journal of Biological Macromolecules·Karima Srih BelghithHafedh Mejdoub

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Citations

Jul 15, 2015·Applied Microbiology and Biotechnology·Wenjing LiWanmeng Mu
Apr 30, 2013·International Journal of Biological Macromolecules·Imen DahechKarima Srih Belghith
Dec 12, 2012·International Journal of Biological Macromolecules·Santhana NakapongPiamsook Pongsawasdi
Jan 11, 2018·Polish Journal of Microbiology·Sahar W M Hassan, Hassan A H Ibrahim
Nov 21, 2018·Bioinspiration & Biomimetics·Joan Combie, Ebru Toksoy Öner
Sep 20, 2017·Plant, Cell & Environment·Maxime VersluysWim Van den Ende
Dec 18, 2019·International Journal of Biological Macromolecules·Amir BouallegueSemia Ellouz-Chaabouni
Apr 7, 2021·Journal of Microbiology and Biotechnology·Safaa A S Al-QaysiMohamed G Farahat

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