PMID: 7400715May 1, 1980

Partial purification of low molecular weight non-suppressible insulin-like activity from human plasma: demonstration of the presence of multiple forms

The Journal of Endocrinology
P N CockerillA C Herington

Abstract

An alternative procedure for the separation of insulin-like growth factors from plasma, avoiding the harsh conditions of acid-ethanol extraction, suggested that several factors which have activity in the isolated rat adipocyte assay may be present. The initial step consisted of ion-exchange chromatography of an enriched Cohn fraction (IV-1) on SP-Sephadix using ammonium acetate buffers. The major activity appeared in a fraction eluted at pH 7.1-9.4. When this fraction was subjected to gel filtration of Sephadix G-75 in 1% formic acid, most of the activity was recovered in a fraction of molecular weight of 5000-8000, but up to 25% of the activity applied remained in a much higher molecular weight form. The active material of low molecular weight was recovered at a considerably greater yield compared with that obtained by the more conventional acid-ethanol extraction followed by gel filtration of Sephadix G-75. The fraction was further purified by DEAE-Sephadix chromatography and isoelectric focussing. Non-suppressible insulin-like activity (NSILA) of pI 4.8 and 6.0 were the major species detected. The results were in contrast with those obtained using acid-ethanol extraction of the Cohn fraction, where the major species were of ...Continue Reading

Citations

Jan 1, 1983·The International Journal of Biochemistry·H J Cornell, A C Herington
Jan 1, 1983·The International Journal of Biochemistry·A C HeringtonA D Kuffer
Jan 1, 1984·Preparative Biochemistry·H J CornellA C Herington
Apr 1, 1989·Journal of Endocrinological Investigation·A Barreca, F Minuto

Related Concepts

Molecular Sieve Chromatography
Ion-Exchange Chromatography Procedure
Isoelectric Focusing
NSILA-S

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