PMID: 6161815Dec 1, 1980Paper

Partial purification of the alpha-tubulin and beta-tubulin messenger RNAs from rat brain

European Journal of Biochemistry
M M PortierF Gros

Abstract

Poly(A)-containing RNA from frozen adult rat brain were fractionated by centrifugation in a formamide/sucrose gradient. Individual fractions were used to program protein synthesis in vitro in a reticulocyte lysate. The cell-free translation products were analyzed by two-dimensional electrophoresis in polyacrylamide slab gels. We observed a heterodispersion of the mRNA translation activity coding for the beta-tubulin subunit which contrasts with a relatively homogeneous distribution of the alpha-tubulin subunit mRNA. These last mRNA species are present in a peak which sediments near the 18-S region of the gradient whereas the beta-tubulin mRNA activity is predominant in the fractions corresponding to the heaviest mRNA species. When these heaviest RNAs were separated again by centrifugation in a second formamide/sucrose gradient, a poly(A)-rich RNA population was obtained that was enriched in RNA for programming the beta-tubulin subunit. Analysis of the products whose synthesis in vitro was directed by this mRNA population revealed that beta tubulin was the main protein formed, the ratio beta/alpha being more than tenfold greater than in the products translated in vitro using total poly(A)-rich RNA.

References

Feb 14, 1979·Biochemical and Biophysical Research Communications·J L Dahl, V J Weibel
May 5, 1979·Brain Research·C A MarottaJ M Gilbert
Dec 1, 1977·FEBS Letters·C Le GuernF Dray
Mar 2, 1978·Nature·R N BryanM Hayashi
Jun 1, 1978·Journal of Neurochemistry·C A MarottaJ M Gilbert
Jul 1, 1978·Experimental Cell Research·J L SaboríoI Mez
Nov 23, 1978·Nature·I Gozes, U Z Littauer
Aug 1, 1976·European Journal of Biochemistry·H R Pelham, R J Jackson
Apr 1, 1977·Journal of Neurochemistry·H FeitF Baskin
Nov 12, 1976·Biochimica Et Biophysica Acta·M A Gilmore-Hebert, S M Heywood
Feb 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·I GozesU Z Littauer
Feb 1, 1976·Nucleic Acids Research·P T Lomedico, G F Saunders
Jan 1, 1976·Analytical Biochemistry·J M Bailey, N Davidson
Aug 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·B A Eipper
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·R F Luduena, D O Woodward
Jan 1, 1973·Annual Review of Biochemistry·J B Olmsted, G G Borisy
Aug 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J Bryan, L Wilson

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