Partial purification of the rat erythrocyte receptor for the channel-forming toxin aerolysin and reconstitution into planar lipid bilayers

Molecular Microbiology
H J GruberJ T Buckley

Abstract

The cytolytic toxin aerolysin binds to a receptor on the surface of eukaryotic cells. Murine erythrocytes are among the most sensitive to the toxin. Here we describe the detergent solubilization and partial purification of the receptor from rat erythrocytes. We show that it can be successfully incorporated into planar lipid bilayers, greatly decreasing the concentration of aerolysin required to form channels. Exploiting the ability of the receptor to bind aerolysin after SDS electrophoresis and blotting, we obtain evidence that it is a 47 kDa glycoprotein that is sensitive to proteases and N-glycosidase. It may correspond to CHIP28, the water channel of the human erythrocyte.

References

Jun 1, 1975·Infection and Immunity·A W BernheimerG Avigad
Jul 8, 1975·Biochemical and Biophysical Research Communications·H FurthmayrV T Marchesi
Jul 27, 1992·FEBS Letters·J T Buckley
Jan 1, 1991·European Biophysics Journal : EBJ·T Schürholz, H Schindler
Jan 1, 1990·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J T Buckley
Jan 1, 1989·Toxicon : Official Journal of the International Society on Toxinology·K M Crowell, F Lutz
Jul 1, 1985·Journal of Bacteriology·S P Howard, J T Buckley
Dec 1, 1973·Analytical Biochemistry·W Schaffner, C Weissmann
May 1, 1973·Analytical Biochemistry·P W Holloway
Oct 1, 1966·Biochemistry·G F SpringerH Tegtmeyer
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·H Schindler, U Quast
Jan 1, 1963·Archives of Biochemistry and Biophysics·J T DODGED J HANAHAN

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Citations

Apr 4, 1998·Electrophoresis·F G van der Goot
Dec 13, 1995·Biochimica Et Biophysica Acta·H J GruberH Schindler
Aug 1, 1997·Current Opinion in Structural Biology·E Gouaux
Apr 1, 1997·Molecular Membrane Biology·C LesieurF Gisou van der Goot
Oct 6, 1999·The Journal of Cell Biology·L Abrami, F G van Der Goot
Jun 6, 1998·Journal of Structural Biology·J RossjohnM W Parker
Sep 10, 1999·The Journal of Biological Chemistry·V M GordonS H Leppla
Jul 11, 1998·The Journal of Biological Chemistry·K H KrauseF G van der Goot
Nov 26, 1998·The Journal of Biological Chemistry·L AbramiF G van der Goot
Jan 30, 1999·Infection and Immunity·J T Buckley, S P Howard

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