Aug 1, 1976

Partial regain of activity in heterologous recombinants of phosphorylcholine-binding M-components from different species

The Journal of Immunology : Official Journal of the American Association of Immunologists
W Riesen

Abstract

Recombination experiments were performed with heavy and light chains derived from a Waldenström's IgM with specificity against phosphorylcholine. The recombinant molecules had an association constant for phosphorylcholine in the same order of magnitude as the native IgM; the number of binding sites at saturation was only slightly decreased in the reconstituted molecules, indicating regain of binding activity after recombination of IgM heavy and light chains. Heterologous recombinants obtained with polypeptide chains of another monoclonal IgM without demonstrable binding activity recovered only 5 to 10% of the binding activity of homologous recombinants. Hybrid molecules prepared with heavy and light chains from the phosphorylcholine-binding mouse IgA myeloma protein TEPC-15, however, regained as much as 41% of the binding activity of the homologous recombinants; these data suggest a considerable degree of structural homology shared by the human IgM and the murine IgA proteins with phosphorylcholine-binding specifity.

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Mentioned in this Paper

Murine
Recombination, Genetic
Polysaccharides, Bacterial
Immunoglobulin Light Chains
Immunoglobulin Heavy Chain Subgroup VH-III
IgM.monoclonal
IgM2
Immunoglobulin A Deficiency (Disorder)
Recombinants
Mus

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